Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo

Ulrike Fischer; Christina Backes; Abdulrahman Raslan; Andreas Keller; Carola Meier; Eckart Meese

doi:10.18632/oncotarget.3248

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo

Ulrike Fischer _, Christina Backes, Abdulrahman Raslan, Andreas Keller, Carola Meier and Eckart Meese

PDF | HTML | Supplementary Files | How to cite

Oncotarget. 2015; 6:7023-7039. https://doi.org/10.18632/oncotarget.3248

Metrics: PDF 2155 views | HTML 2243 views | ?

Abstract

Ulrike Fischer1, Christina Backes1,3, Abdulrahman Raslan2, Andreas Keller3, Carola Meier2, Eckart Meese1

1Department of Human Genetics, Saarland University, 66421 Homburg/Saar, Germany

2Department of Anatomy and Cell Biology, Saarland University, 66421 Homburg/Saar, Germany

3Clinical Bioinformatics, Saarland University, 66123 Saarbrücken, Germany

Correspondence to:

Ulrike Fischer, e-mail: [email protected]

Keywords: neurosphere, genomic stability, in vivo, array-CGH

Received: November 04, 2014 Accepted: January 28, 2015 Published: February 19, 2015

ABSTRACT

In development of amphibians and flies, gene amplification is one of mechanisms to increase gene expression. In mammalian cells, gene amplification seems to be restricted to tumorigenesis and acquiring of drug-resistance in cancer cells. Here, we report a complex gene amplification pattern in mouse neural progenitor cells during differentiation with approximately 10% of the genome involved. Half of the amplified mouse chromosome regions overlap with amplified regions previously reported in human neural progenitor cells, indicating conserved mechanisms during differentiation. Using fluorescence in situ hybridization, we verified the amplification in single cells of primary mouse mesencephalon E14 (embryonic stage) neurosphere cells during differentiation. In vivo we confirmed gene amplifications of the TRP53 gene in cryosections from mouse embryos at stage E11.5. Gene amplification is not only a cancer-related mechanism but is also conserved in evolution, occurring during differentiation of mammalian neural stem cells

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 3248

Publication Alerts

Research Papers:

Gene amplification during differentiation of mammalian neural stem cells in vitro and in vivo

Abstract