MiR-34a suppresses amphiregulin and tumor metastatic potential of head and neck squamous cell carcinoma (HNSCC)
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Jiali Zhang1,2,*, Yu Wang1,2*, Xinming Chen1,2, Yi Zhou1, Fangyan Jiang1, Jirong Chen1, Li Wang1, Wen-Feng Zhang1
1The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei_MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China
2Oral Histopathology Department, School and Hospital of Stomatology, Wuhan University, Wuhan, China
*These authors have contributed equally to this work
Jiali Zhang, e-mail: firstname.lastname@example.org
Xinming Chen, e-mail: email@example.com
Wen-Feng Zhang, e-mail: firstname.lastname@example.org
Keywords: Head and neck squamous cell carcinoma, miR-34a, amphiregulin, metastasis
Received: August 11, 2014 Accepted: January 16, 2015 Published: February 05, 2015
MiR-34a is a well-known tumor metastasis inhibitor, but only a few target genes involved in metastasis have been identified. In HNSCC, the role of miR-34a in metastasis has not been fully elaborated, and the target gene of miR-34a is still blind. Here we addressed that, the relative lower expression of miR-34a is associated with HNSCC lymphatic metastasis. HNSCC metastasis was found to be strongly suppressed in vitro and in vivo by over-expressing miR-34a. In order to screen the possible target genes of miR-34a in HNSCC, a microarray-based differential mRNA profiling mediated by miR-34a over-expression was performed, and AREG was identified as a pivotal target. We demonstrated that the mRNA and protein levels of AREG were greatly reduced when forcing miR-34a expression. The correlation between AREG mRNA levels and HNSCC metastatic phenotype was also significant in HNSCC tissues (p < 0.01). Moreover, the results of luciferase assay provided the further evidence that miR-34a degraded AREG mRNA through targeting the 3’-UTR site. Restoration of AREG expression partially rescued miR-34a-mediated cell invasion defects in vivo and in vitro. Additionally, Over-expressing miR-34a greatly reduced EGFR and uPA, which were reversed by re-expression of AREG. Taken together, these findings indicate that miR-34a targets AREG, and is essential in inhibition of HNSCC metastasis.
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