Oncotarget

Research Papers:

Involvement of Akt-1 and mTOR in Sensitivity of Breast Cancer to Targeted Therapy

Melissa L. Sokolosky, Kristin M. Stadelman, William H. Chappell, Stephen L. Abrams, Alberto M. Martelli, Franca Stivala, Massimo Libra, Ferdinando Nicoletti, Lyudmyla B. Drobot, Richard A. Franklin, Linda S. Steelman and James A. McCubrey _

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Oncotarget. 2011; 2:538-550. https://doi.org/10.18632/oncotarget.302

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Abstract

Melissa L. Sokolosky1,2, Kristin M. Stadelman1,2, William H. Chappell1, Stephen L. Abrams1, Alberto M. Martelli3,4, Franca Stivala5, Massimo Libra5, Ferdinando Nicoletti5, Lyudmyla B. Drobot6, Richard A. Franklin1 Linda S. Steelman1, and James A. McCubrey1

1Department of Microbiology & Immunology Brody School of Medicine at East Carolina University Greenville, NC 27858 USA

2These two authors contributed equally to the studies.

3Dipartimento di Scienze Anatomiche Umane e Fisiopatologia dell'Apparato Locomotore Università di Bologna, Bologna, Italy

4 IGM-CNR, Sezione di Bologna, C/o IOR, Bologna, Italy

5Department of Biomedical Sciences University of Catania, Catania, Italy

6Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Received: June 23, 2011; Accepted: July 1, 2011; Published: July 1, 2011;

Keywords: Akt, mTOR, Targeted Therapy, Drug Resistance

Correspondence:

James A. McCubrey, email:

Abstract

Elucidating the response of breast cancer cells to chemotherapeutic and hormonal based drugs is clearly important as these are frequently used therapeutic approaches. A signaling pathway often involved in chemo- and hormonal-resistance is the Ras/PI3K/PTEN/Akt/mTOR cascades. In the studies presented in this report, we have examined the effects of constitutive activation of Akt on the sensitivity of MCF-7 breast cancer cells to chemotherapeutic- and hormonal-based drugs as well as mTOR inhibitors. MCF-7 cells which expressed a constitutively-activated Akt-1 gene [∆Akt-1(CA)] were more resistant to doxorubicin, etoposide and 4-OH-tamoxifen (4HT) than cells lacking ∆Akt-1(CA). Cells which expressed ∆Akt-1(CA) were hypersensitive to the mTOR inhibitor rapamycin. Furthermore, rapamycin lowered the IC50s for doxorubicin, etoposide and 4HT in the cells which expressed ∆Akt-1(CA), demonstrating a potential improved method for treating certain breast cancers which have deregulated PI3K/PTEN/Akt/mTOR signaling. Understanding how breast cancers respond to chemo- and hormonal-based therapies and the mechanisms by which they can become drug resistant may enhance our ability to treat breast cancer. These results also document the potential importance of knowledge of the mutations present in certain cancers which may permit more effective therapies.


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