Oncotarget

Research Papers:

Functional interplay between YY1 and CARM1 promotes oral carcinogenesis

Amit K. Behera, Manoj Kumar, Muthu K. Shanmugam, Aditya Bhattacharya, Vinay J. Rao, Akshay Bhat, Madavan Vasudevan, Kodaganur S. Gopinath, Azeem Mohiyuddin, Anupam Chatterjee, Gautam Sethi _ and Tapas K. Kundu _

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Oncotarget. 2019; 10:3709-3724. https://doi.org/10.18632/oncotarget.26984

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Abstract

Amit K. Behera1, Manoj Kumar1, Muthu K. Shanmugam2, Aditya Bhattacharya1, Vinay J. Rao1, Akshay Bhat1, Madavan Vasudevan3, Kodaganur S. Gopinath4, Azeem Mohiyuddin5,6,7, Anupam Chatterjee8, Gautam Sethi2 and Tapas K. Kundu1

1 Transcription and Disease Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Bangalore 560064, India

2 Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore 117600, Singapore

3 Bionivid Technology Private Limited, Kasturi Nagar, Bangalore 560043, India

4 Department of Surgical Oncology, HCG Bangalore Institute of Oncology, Bangalore 560027, India

5 Department of Pathology, Sri Devaraj Urs Academy of Higher Education and Research Center, Kolar, Bangalore 563101, India

6 Department of Ear, Nose and Throat, Sri Devaraj Urs Academy of Higher Education and Research Center, Kolar, Bangalore 563101, India

7 Department of Head and Neck Surgery, Sri Devaraj Urs Academy of Higher Education and Research Center, Kolar, Bangalore 563101, India

8 Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong, Meghalaya 793022, India

Correspondence to:

Tapas K. Kundu,email: tapas@jncasr.ac.in
Gautam Sethi,email: phcgs@nus.edu.sg

Keywords: oral cancer; oncogene; YY1; CARM1; arginine methylation

Received: January 19, 2019     Accepted: May 13, 2019     Published: June 04, 2019

ABSTRACT

Coactivator associated arginine methyltransferase 1 (CARM1) has been functionally implicated in maintenance of pluripotency, cellular differentiation and tumorigenesis; where it plays regulatory roles by virtue of its ability to coactivate transcription as well as to modulate protein function as an arginine methyltransferase. Previous studies establish an oncogenic function of CARM1 in the context of colorectal and breast cancer, which correlate to its overexpressed condition. However, the mechanism behind its deregulated expression in the context of cancer has not been addressed before. In the present study we uncover an oncogenic function of CARM1 in the context of oral cancer, where it was found to be overexpressed. We also identify YY1 to be a positive regulator of CARM1 gene promoter, where silencing of YY1 in oral cancer cell line could lead to reduction in expression of CARM1. In this context, YY1 showed concomitant overexpression in oral cancer patient samples compared to adjacent normal tissue. Cell line based experiments as well as xenograft study revealed pro-neoplastic functions of YY1 in oral cancer. Transcriptomics analysis as well as qRT-PCR validation clearly indicated pro-proliferative, pro-angiogenic and pro-metastatic role of YY1 in oral cancer. We also show that YY1 is a substrate of CARM1 mediated arginine methylation, where the latter could coactivate YY1 mediated reporter gene activation in vivo. Taken together, CARM1 and YY1 were found to regulate each other in a positive feedback loop to facilitate oral cancer progression.


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