Oncotarget

Research Papers:

A three-step approach identifies novel shear stress-sensitive endothelial microRNAs involved in vasculoprotective effects of high-intensity interval training (HIIT)

Boris Schmitz, Franziska L. Breulmann, Bothaynah Jubran, Florian Rolfes, Lothar Thorwesten, Michael Krüger, Andreas Klose, Hans-Joachim Schnittler and Stefan-Martin Brand

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Oncotarget. 2019; 10:3625-3640. https://doi.org/10.18632/oncotarget.26944

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Abstract

Boris Schmitz1,*, Franziska L. Breulmann1,*, Bothaynah Jubran2, Florian Rolfes1, Lothar Thorwesten1, Michael Krüger3, Andreas Klose3, Hans-Joachim Schnittler2 and Stefan-Martin Brand1

1 Institute of Sports Medicine, Molecular Genetics of Cardiovascular Disease, University Hospital Muenster, Muenster, Germany

2 Institute of Anatomy and Vascular Biology, University of Muenster, Muenster, Germany

3 Department of Physical Education and Sports History, University of Muenster, Muenster, Germany

* These authors contributed equally to this work

Correspondence to:

Boris Schmitz,email: boris.schmitz@ukmuenster.de

Keywords: miRNA; shear stress; performance; endothelium; high-intensity training (HIT)

Received: November 17, 2018     Accepted: April 03, 2019     Published: June 04, 2019

ABSTRACT

Circulatory microRNAs (c-miRNAs) are regulated in response to physical activity and may exert anti-atherosclerotic effects. Since the vascular endothelium is an abundant source of c-miRNAs, we aimed to identify novel vasculoprotective exercise-induced c-miRNAs by the combined analysis of published endothelial miRNA array data followed by in vivo and in vitro validation. We identified 8 different array-based publications reporting 185 endothelial shear stress-regulated miRNAs of which 13 were identified in ≥3 independent reports. Nine miRNAs had already been associated with physical activity. Of the remaining novel miRNAs, miR-98-3p and miR-125-5p were selected for further analysis due to reported vasculoprotective effects. Analysis in two different 4-week high-intensity interval training (HIIT) groups (group 1 [n=27]: 4x30 s, group 2 [n=25]: 8x15 s; all-out running) suggested significantly elevated miR-98 and miR-125a-5p levels in response to acute exercise at baseline and at follow-up. Endothelial in vitro shear stress experiments revealed increased miR-125a-5p and miR-98-3p levels in medium of human umbilical vein endothelial cells at 30 dyn/cm2 after 20 and 60 min, respectively. Our results suggest that miR-98-3p and miR-125a-5p can be rapidly secreted by endothelial cells, which might be the source of increased c-miR-98-3p and -125a-5p levels in response to HIIT. Both miRNAs attenuate endothelial inflammation and may mediate vasculoprotective effects of physical exercise including HIIT.


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