Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs
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Qi Zhang1,2,*, Luxia Jiang3,*, Jing Yu4, Limin Tian1, Tiankang Guo1, Baoshan Di5, Jinxing Quan1, Jing Feng1 and Jing Liu1
1 Department of Endocrinology, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China
2 School of Basic Medical Sciences, Lanzhou University, Lanzhou City 730000, Gansu Province, China
3 Intensive Care Unit, Lanzhou University Second Hospital, Lanzhou City 730000, Gansu Province, China
4 Gansu University of Chinese Medicine, Lanzhou City 730000, Gansu Province, China
5 Emergency Department, Gansu Provincial Hospital, Lanzhou City 730000, Gansu Province, China
* These authors contributed equally to this work
Keywords: human liver sinusoidal endothelial cells; integrin αvβ3; laminin
Received: September 14, 2017 Accepted: March 24, 2018 Published: June 25, 2019
To investigate the roles of reactive oxygen species (ROS) and integrin αvβ3 in palmitate-induced laminin expression of human liver sinusoidal endothelial cells (HLSECs).
The protein expression of integrin αv, integrin β3 and laminin are increased by palmitate in HLSECs in a time- and dose-dependent manner. NAC, the ROS inhibitor, significantly inhibited the up-regulation of protein expression of integrin αv, integrin β3 and laminin by palmitate (P < 0.05). Palmitate markedly enhanced ROS formation (P < 0.05), which was not inhibited by LM609, the antibody of integrin αvβ3. Palmitate significantly increased laminin synthesis (P < 0.05), which was attenuated by LM609 and NAC (P < 0.05).
Materials and Methods
HLSECs were treated with palmitate in the presence or absence of LM609 (10 μg/ml) or N-acetylcysteine (NAC) (2 mM). Expression of integrin αv, integrin β3 and laminin were measured by RT-PCR and Western blot. Immunocytochemistry were used for examining laminin expression. The generation of ROS was measured using the fluorescent signal 2',7' dichloro-fluorescein diacetate (DCFH-DA).
The results suggested that palmitate increases laminin expression through ROS/integrin αv/β3 pathway.
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