The assessment of correlation and prognosis among 18F-FDG uptake parameters, Glut1, pStat1 and pStat3 in surgically resected non-small cell lung cancer patients
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Hayato Kaida1, Koichi Azuma2, Akihiko Kawahara3, Eiji Sadashima4, Satoshi Hattori5, Shinzo Takamori6, Jun Akiba3, Kiminori Fujimoto7, Axel Rominger8, Takamichi Murakami9, Kazunari Ishii1 and Masatoshi Ishibashi10
1Department of Radiology, Kindai University Faculty of Medicine, Osakasayama, Osaka, Japan
2Division of Respirology, Department of Internal Medicine, Kurume University School of Medicine, Kurume, Fukuoka, Japan
3Department of Diagnostic Pathology, Kurume University Hospital, Kurume, Fukuoka, Japan
4Life Science, Saga-Ken Medical Centre Koseikan, Saga, Saga, Japan
5Department of Biomedical Statistics, Osaka University Graduate School of Medicine, Suita, Osaka, Japan
6Department of Surgery, Kurume University School of Medicine, Kurume, Fukuoka, Japan
7Department of Radiology, Kurume University School of Medicine, Kurume, Fukuoka, Japan
8Department of Nuclear Medicine, Inselspital, Bern University Hospital, Bern, Switzerland
9Department of Radiology, Kobe University Graduate School of Medicine, Kobe, Hyogo, Japan
10Department of Radiology, Fukuoka Tokushukai Medical Center, Kasuga, Fukuoka, Japan
Hayato Kaida, email: email@example.com
Keywords: non-small cell lung cancer; volumetric parameters; Glut1; pStat1; pStat3
Received: June 09, 2018 Accepted: July 13, 2018 Published: August 10, 2018
Introduction: To assess the correlation among 18F-FDG uptake, Glut1, pStat1 and pStat3, and to investigate the relationship between the prognosis and 18F-FDG uptake and these molecular markers in surgically resected non-small cell lung cancer (NSCLC) patients.
Results: Knockdown of Glut1 led to a significant increase in pStat1 expression. Glut1 expression positively correlated with the SUVmax, SUVmean, and TLG significantly (P<0.001). pStat3 expression negatively correlated with all PET parameters significantly (P<0.001). pStat1 had positive weak correlations with the SUVmax and SUVmean. All PET parameters and Glut1 were significantly associated with DFS (P<0.05). TLG, MTV, Glut1 and pStat1 were significantly associated with OS (P<0.05).
Conclusion: pStat3 and Glut1 may be associated with 18F-FDG uptake mechanism. TLG, MTV, and Glut1 may be independent prognostic factors.
Methods: The SUVmax, SUVmean, MTV and TLG of primary lesions were calculated in 140 patients. The expressions of Glut1 and Stat pathway proteins in NSCLC cell lines were examined by immune blots. Excised tumor tissue was analyzed by immunohistochemistry. OS and DFS were evaluated by the Kaplan-Meier method. The difference in survival between subgroups was analyzed by log-rank test. The prognostic significance of clinicopathological, molecular and PET parameters was assessed by Cox proportional hazard regression analysis.
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