Oncotarget

Research Papers:

Differential miRNA expression profiling reveals miR-205-3p to be a potential radiosensitizer for low- dose ionizing radiation in DLD-1 cells

Rodrigo Andaur, Julio C. Tapia, José Moreno, Leopoldo Soto, Ricardo Armisen and Katherine Marcelain _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2018; 9:26387-26405. https://doi.org/10.18632/oncotarget.25405

Metrics: PDF 360 views  |   HTML 909 views  |   ?  


Abstract

Rodrigo Andaur1, Julio C. Tapia1,5, José Moreno2,3,4, Leopoldo Soto2,3,4, Ricardo Armisen1,5,6 and Katherine Marcelain1,5

1Departamento de Oncología Básico-Clínica, Facultad de Medicina, Universidad de Chile, Santiago, Chile

2Comisión Chilena de Energía Nuclear, Santiago, Chile

3Center for Research and Applications in Plasma Physics and Pulsed Power, P4, Talca, Chile

4Departamento de Ciencias Físicas, Universidad Andres Bello, Santiago, Chile

5Centro de Investigación y Tratamiento del Cáncer, Facultad de Medicina, Universidad de Chile, Santiago, Chile

6Current Address: Center of Excellence in Precision Medicine, Pfizer, Santiago, Chile

Correspondence to:

Katherine Marcelain, email: kmarcelain@med.uchile.cl

Keywords: radiosensitivity; low-dose ionizing radiation; miRNA; miR-205-3p

Received: June 23, 2017     Accepted: April 28, 2018     Published: May 29, 2018

ABSTRACT

Enhanced radiosensitivity at low doses of ionizing radiation (IR) (0.2 to 0.6 Gy) has been reported in several cell lines. This phenomenon, known as low doses hyper-radiosensitivity (LDHRS), appears as an opportunity to decrease toxicity of radiotherapy and to enhance the effects of chemotherapy. However, the effect of low single doses IR on cell death is subtle and the mechanism underlying LDHRS has not been clearly explained, limiting the utility of LDHRS for clinical applications. To understand the mechanisms responsible for cell death induced by low-dose IR, LDHRS was evaluated in DLD-1 human colorectal cancer cells and the expression of 80 microRNAs (miRNAs) was assessed by qPCR array. Our results show that DLD-1 cells display an early DNA damage response and apoptotic cell death when exposed to 0.6 Gy. miRNA expression profiling identified 3 over-expressed (miR-205-3p, miR-1 and miR-133b) and 2 down-regulated miRNAs (miR-122-5p, and miR-134-5p) upon exposure to 0.6 Gy. This miRNA profile differed from the one in cells exposed to high-dose IR (12 Gy), supporting a distinct low-dose radiation-induced cell death mechanism. Expression of a mimetic miR-205-3p, the most overexpressed miRNA in cells exposed to 0.6 Gy, induced apoptotic cell death and, more importantly, increased LDHRS in DLD-1 cells. Thus, we propose miR-205-3p as a potential radiosensitizer to low-dose IR.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 25405