Deregulation of methylation of transcribed-ultra conserved regions in colorectal cancer and their value for detection of adenomas and adenocarcinomas
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Anastasia E. Kottorou1, Anna G. Antonacopoulou1, Foteinos-Ioannis D. Dimitrakopoulos1,3, Georgia Diamantopoulou2, Chaido Sirinian1, Melpomeni Kalofonou1,6, Theodoros Theodorakopoulos2, Chrysa Oikonomou3, Evangelos C. Katsakoulis2, Angelos Koutras1,3, Thomas Makatsoris1,3, Nikos Demopoulos4, Georgia Stephanou4, Michalis Stavropoulos5, Konstantinos C. Thomopoulos2 and Haralabos P. Kalofonos1,3
1Clinical and Molecular Oncology Laboratory, Division of Oncology, Medical School, University of Patras, Patras, Greece
2Division of Gastroenterology, University Hospital of Patras, Patras, Greece
3Division of Oncology, University Hospital of Patras, Patras, Greece
4Division of Genetics, Cell and Developmental Biology, Department of Biology, University of Patras, Patras, Greece
5Department of Surgery, Medical School, University of Patras, Patras, Greece
6Institute of Biomedical Engineering, Imperial College London, London, UK
Haralabos P. Kalofonos, email: firstname.lastname@example.org
Keywords: transcribed-ultra conserved regions; colorectal cancer and adenomas; tissue and plasma methylation; screening biomarker; expression levels
Received: January 12, 2017 Accepted: March 02, 2018 Published: April 20, 2018
Expression of Transcribed Ultraconserved Regions (T-UCRs) is often deregulated in cancer. The present study assesses the expression and methylation of three T-UCRs (Uc160, Uc283 and Uc346) in colorectal cancer (CRC) and explores the potential of T-UCR methylation in circulating DNA for the detection of adenomas and adenocarcinomas.
Expression levels of Uc160, Uc283 and Uc346 were lower in neoplastic tissues from 64 CRC patients (statistically significant for Uc160, p<0.001), compared to non-malignant tissues, while methylation levels displayed the inverse pattern (p<0.001, p=0.001 and p=0.004 respectively). In colon cancer cell lines, overexpression of Uc160 and Uc346 led to increased proliferation and migration rates. Methylation levels of Uc160 in plasma of 50 CRC, 59 adenoma patients, 40 healthy subjects and 12 patients with colon inflammation or diverticulosis predicted the presence of CRC with 35% sensitivity and 89% specificity (p=0.016), while methylation levels of the combination of all three T-UCRs resulted in 45% sensitivity and 74.3% specificity (p=0.013). In conclusion, studied T-UCRs’ expression and methylation status are deregulated in CRC while Uc160 and Uc346 appear to have a complicated role in CRC progression. Moreover their methylation status appears a promising non-invasive screening test for CRC, provided that the sensitivity of the assay is improved.
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