Oncotarget

Research Papers:

A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids

Yu-Hsiu Su, Tai-Yun Lin, Hung-Jen Liu and Chin-Kai Chuang _

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Oncotarget. 2018; 9:20490-20507. https://doi.org/10.18632/oncotarget.24960

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Abstract

Yu-Hsiu Su1,2, Tai-Yun Lin1, Hung-Jen Liu2,3,4 and Chin-Kai Chuang1

1Division of Biotechnology, Animal Technology Laboratories, Agricultural Technology Research Institute, Hsinchu City 30093, Taiwan

2Institute of Molecular Biology, National Chung Hsing University, Taichung City 40227, Taiwan

3Rong Hsing Research Center for Translational Medicine, National Chung Hsing University, Taichung City 40227, Taiwan

4The iEGG and Animal Biotechnology Center, National Chung Hsing University, Taichung City 40227, Taiwan

Correspondence to:

Chin-Kai Chuang, email: jkjuang@mail.atri.org.tw

Hung-Jen Liu, email: hjliu5257@nchu.edu.tw

Keywords: cancer stem cells; homing peptides; glycosphingolipids; Globo H; Lewis Y

Received: February 15, 2017     Accepted: March 12, 2018     Published: April 17, 2018

ABSTRACT

Cancer stem cells (CSCs) are currently believed to be involved in tumor metastasis and relapse. And treatments against CSCs are well concerned issues. Peptides targeting to mouse and human CSCs were screened from an M13 phage display library. The first subset of cancer stem cell homing peptides (CSC HPs), CSC HP-1 to -12, were screened with mouse EMT6 breast cancer stem cells. Among them, CSC HP-1, CSC HP-3, CSC HP-8, CSC HP-9, and CSC HP-10 can bind to mouse CT26 colon CSCs; CSC HP-1, CSC HP-2, CSC HP-3, and CSC HP-8 can bind to mouse Hepa1-6 liver CSCs; as well as CSC HP-1, CSC HP-2, CSC HP-3, CSC HP-8, CSC HP-9, CSC HP-10, and CSC HP-11 can bind to human PANC-1 pancreatic CSCs. The second subset of cancer stem cell homing peptides, CSC HP-hP1 to -hP3, were screened with human PANC-1 pancreatic CSCs. Both CSC HP-hP1 and CSC HP-hP2 were demonstrated able to bind mouse EMT6, CT26 and Hepa1-6 CSCs as well as human colorectal HT29 and lung H1650 CSCs. CSC HP-1 and CSC HP-hP1 could strongly associate with the Globo 4 and Lewis Y glycan epitopes coupled on a microarray chip or Globo 4 and Globo H conjugated on bovine serum albumin. CSC HP-10, CSC HP-11 and CSC HP-hP2 could associate with the disialylated saccharide Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc coupled on a microarray chip. These results indicate that the CSC HPs may target to the known stem cell glycan markers GbH and Lewis Y as well as the disialylated saccharide.


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