Oncotarget

Research Papers:

Characterization of brain tumor initiating cells isolated from an animal model of CNS primitive neuroectodermal tumors

Sergey Malchenko _, Simone T. Sredni, Jerusha Boyineni, Yingtao Bi, Naira V. Margaryan, Maheedhara R. Guda, Yulia Kostenko, Tadanori Tomita, Ramana V. Davuluri, Kiran Velpula, Mary J.C. Hendrix and Marcelo B. Soares

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Oncotarget. 2018; 9:13733-13747. https://doi.org/10.18632/oncotarget.24460

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Abstract

Sergey Malchenko1, Simone T. Sredni2,3,*, Jerusha Boyineni1,*, Yingtao Bi4,5, Naira V. Margaryan6, Maheedhara R. Guda1, Yulia Kostenko1, Tadanori Tomita2, Ramana V. Davuluri4, Kiran Velpula1, Mary J.C. Hendrix6,7 and Marcelo B. Soares1

1Department of Cancer Biology & Pharmacology, University of Illinois College of Medicine, Peoria, Illinois, United States of America

2Department of Surgery, Division of Pediatric Neurosurgery, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America

3Cancer Biology and Epigenomics Program at The Stanley Manne Children’s Research Institute, Ann & Robert H. Lurie Children’s Hospital of Chicago, Chicago, Illinois, United States of America

4Department of Preventive Medicine, Division of Health and Biomedical Informatics, Feinberg School of Medicine, Northwestern University, Chicago, Illinois, United States of America

5AbbVie Bioresearch Center, Worcester, Massachusetts, United States of America

6Department of Biochemistry, Robert C. Byrd Health Sciences Center and Cancer Institute, West Virginia University, Morgantown, West Virginia, United States of America

7Department of Biology, Shepherd University, Shepherdstown, West Virginia, United States of America

*These authors have contributed equally to this work

Correspondence to:

Sergey Malchenko, email: sergeynm@uicomp.uic.edu

Keywords: CNS-PNET animal model; radial glia; brain tumor-initiating cells; RNA/DNA-seq; gene signature

Received: December 10, 2017    Accepted: January 30, 2018    Published: February 09, 2018

ABSTRACT

CNS Primitive Neuroectodermal tumors (CNS-PNETs) are members of the embryonal family of malignant childhood brain tumors, which remain refractory to current therapeutic treatments. Current paradigm of brain tumorigenesis implicates brain tumor-initiating cells (BTIC) in the onset of tumorigenesis and tumor maintenance. However, despite their significance, there is currently no comprehensive characterization of CNS-PNETs BTICs. Recently, we described an animal model of CNS-PNET generated by orthotopic transplantation of human Radial Glial (RG) cells - the progenitor cells for adult neural stem cells (NSC) - into NOD-SCID mice brain and proposed that BTICs may play a role in the maintenance of these tumors. Here we report the characterization of BTIC lines derived from this CNS-PNET animal model. BTIC’s orthotopic transplantation generated highly aggressive tumors also characterized as CNS-PNETs. The BTICs have the hallmarks of NSCs as they demonstrate self-renewing capacity and have the ability to differentiate into astrocytes and early migrating neurons. Moreover, the cells demonstrate aberrant accumulation of wild type tumor-suppressor protein p53, indicating its functional inactivation, highly up-regulated levels of onco-protein cMYC and the BTIC marker OCT3/4, along with metabolic switch to glycolysis - suggesting that these changes occurred in the early stages of tumorigenesis. Furthermore, based on RNA- and DNA-seq data, the BTICs did not acquire any transcriptome-changing genomic alterations indicating that the onset of tumorigenesis may be epigenetically driven. The study of these BTIC self-renewing cells in our model may enable uncovering the molecular alterations that are responsible for the onset and maintenance of the malignant PNET phenotype.


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