The long non-coding RNA SNHG14 inhibits cell proliferation and invasion and promotes apoptosis by sponging miR-92a-3p in glioma
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Qiang Wang1,*, Yiwan Teng2,*, Rong Wang3, Danni Deng3, Yijie You1, Ya Peng1, Naiyuan Shao1 and Feng Zhi1,3
1Department of Neurosurgery, The First People’s Hospital of Changzhou, Changzhou, Jiangsu, China
2Changzhou Center for Biotech Development, Changzhou, Jiangsu, China
3Modern Medical Research Center, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu, China
*These authors contributed equally to this work
Ya Peng, email: email@example.com
Naiyuan Shao, email: firstname.lastname@example.org
Feng Zhi, email: email@example.com
Keywords: SNHG14; miR-92a-3p; glioma; prolifertion; invasion
Received: July 29, 2017 Accepted: November 16, 2017 Published: January 04, 2018
Malignant glioma is one of the most common types of primary brain tumours. Long non-coding RNAs (lncRNAs) have recently emerged as a new class of therapeutic targets for many cancers. In this study, we aimed to explore the functional involvement of small nucleolar RNA host gene 14 (SNHG14) and its potential regulatory mechanism in glioma progression. SNHG14 was found to be downregulated in human glioma tissues and cell lines. SNHG14 significantly inhibited cell viability, reduced cell invasion, and induced apoptosis in glioma cell lines. Furthermore, a correlation analysis demonstrated that there was a negative correlation between SNHG14 expression and miR-92a-3p expression. Bioinformatics prediction and luciferase reporter assays demonstrated that miR-92a-3p could directly bind to SNHG14. miR-92a-3p was significantly upregulated in glioma and acted as an oncogene in glioma cells by inhibiting Bim. Moreover, mechanistic investigations showed that miR-92a-3p could reverse the tumour suppressive effects induced by SNHG14 in glioma, indicating that SNHG14 may act as an endogenous sponge that competes for binding to miR-92a-3p. Our results suggest that SNHG14 and miR-92a-3p may be promising molecular targets for glioma therapy.
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