Oncotarget

Research Papers:

The single-nucleotide polymorphisms in CHD5 affect the prognosis of patients with hepatocellular carcinoma

Xiao Zhu _, Qingming Kong, Liwei Xie, Zhihong Chen, Hongmei Li, Zhu Zhu, Yongmei Huang, Feifei Lan, Haiqing Luo, Jingting Zhan, Hongrong Ding, Jinli Lei, Qin Xiao, Weiming Fu, Wenguo Fan, Jinfang Zhang and Hui Luo

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Oncotarget. 2018; 9:13222-13230. https://doi.org/10.18632/oncotarget.23812

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Abstract

Xiao Zhu1,10,11,*, Qingming Kong2,*, Liwei Xie3,*, Zhihong Chen1,*, Hongmei Li1, Zhu Zhu1, Yongmei Huang1,4, Feifei Lan5, Haiqing Luo1,6, Jingting Zhan1,4, Hongrong Ding1, Jinli Lei1,4, Qin Xiao7, Weiming Fu8, Wenguo Fan9, Jinfang Zhang12 and Hui Luo1,4

1Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics, Dongguan Scientific Research Center, Guangdong Medical University, Dongguan, China

2Immunity and Biochemical Research Lab, Zhejiang Academy of Medical Sciences, Hangzhou, China

3Guangdong Institute of Microbiology, Guangzhou, China

4Institute of Marine Medicine Research, Guangdong Medical University, Zhanjiang, China

5Forensic Identification Institute, Guangdong Women and Children Hospital, Guangzhou, China

6The Affiliated Hospital Cancer Center, Guangdong Medical University, Zhanjiang, China

7Department of Blood Transfusion, Peking University Shenzhen Hospital, Shenzhen, China

8School of Pharmaceutical Sciences, Southern Medical University, Guangzhou, Guangdong, China

9Guanghua School of Stomatology, Hospital of Stomatology, Sun Yat-sen University, Guangzhou, China

10Cancer Center, Medical College of Georgia, Georgia Health Sciences University, Augusta, GA, USA

11Institute of Bioinformatics, University of Georgia, Athens, GA, USA

12Key Laboratory of Orthopaedics and Traumatology, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, The First Clinical Medical College, Guangzhou University of Chinese Medicine, Guangzhou, China

*These authors contributed equally to this work

Correspondence to:

Hui Luo, email: luohui@gdmu.edu.cn

Jinfang Zhang, email: zhangjf06@cuhk.edu.hk

Keywords: hepatocellular carcinoma; chromodomain-helicase-DNA-binding protein 5; prognosis; single-nucleotide polymorphisms; linkage disequilibrium

Received: May 27, 2017     Accepted: November 19, 2017     Published: December 26, 2017

ABSTRACT

Previous studies showed that the low expressions of chromodomain-helicase-DNA-binding protein 5 (CHD5) were intensively associated with deteriorative biologic and clinical characteristics as well as outcomes in many tumors. The aim of this study is to determine whether CHD5 single nucleotide polymorphisms (SNPs) contribute to the prognosis of hepatocellular carcima (HCC). The SNPs were selected according to their linkage disequilibrium (LD) in the targeted next-generation sequencing (NGS) and then genotyped with TaqMan probers. We revealed a rare haplotype AG in CHD5 (SNPs: rs12564469-rs9434711) was markedly associated with HCC prognosis. The univariate and multivariate regression analyses revealed the patients with worse overall survival time were those with tumor metastasis and haplotype AG, as well as cirrhosis, poor differentiation and IV-TNM stage. Based on the available public databases, we discovered the significant association between haplotype AG and CHD5 mRNA expressions only existed in Chinese. These data proposed that the potentially genetic haplotype might functionally contribute to HCC prognosis and CHD5 mRNA expressions.


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