Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Effect of the diet type and temperature on the C. elegans transcriptome

Eva Gomez-Orte, Eric Cornes, Angelina Zheleva, Beatriz Sáenz-Narciso, María de Toro, María Iñiguez, Rosario Lopez, Juan-Félix San-Juan, Begoña Ezcurra, Begoña Sacristán, Adolfo Sánchez-Blanco, Julián Cerón and Juan Cabello _

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Oncotarget. 2018; 9:9556-9571. https://doi.org/10.18632/oncotarget.23563

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Abstract

Eva Gómez-Orte1, Eric Cornes2,6, Angelina Zheleva1, Beatriz Sáenz-Narciso1, María de Toro1, María Iñiguez1, Rosario López1, Juan-Félix San-Juan3, Begoña Ezcurra1, Begoña Sacristán4, Adolfo Sánchez-Blanco5, Julián Cerón2 and Juan Cabello1

1CIBIR (Center for Biomedical Research of La Rioja), Logroño, 26006 La Rioja, Spain

2Bellvitge Biomedical Research Institute - IDIBELL, L’Hospitalet de Llobregat, 08908 Barcelona, Spain

3University of La Rioja, Logroño, 26006 La Rioja, Spain

4Hospital San Pedro, Logroño, 26006 La Rioja, Spain

5Department of Biology, University of Hartford, West Hartford, 06117 CT, USA

6Present address: Institut Pasteur, 75015 Paris, France

Correspondence to:

Juan Cabello, email: juan.cabello@riojasalud.es

Keywords: transcriptomic analysis; physiological response; growth temperature; diet; caenorhabditis elegans; Gerotarget

Received: July 29, 2017     Accepted: October 27, 2017     Published: December 21, 2017

ABSTRACT

The transcriptomes of model organisms have been defined under specific laboratory growth conditions. The standard protocol for Caenorhabditis elegans growth and maintenance is 20ºC on an Escherichia coli diet. Temperatures ranging from 15ºC to 25°C or feeding with other species of bacteria are considered physiological conditions, but the effect of these conditions on the worm transcriptome has not been well characterized. Here, we compare the global gene expression profile for the reference Caenorhabditis elegans strain (N2) grown at 15°C, 20°C, and 25°C on two different diets, Escherichia coli and Bacillus subtilis. When C. elegans were fed E. coli and the growth temperature was increased, we observed an enhancement of defense response pathways and down-regulation of genes associated with metabolic functions. However, when C. elegans were fed B. subtilis and the growth temperature was increased, the nematodes exhibited a decrease in defense response pathways and an enhancement of expression of genes associated with metabolic functions. Our results show that C. elegans undergo significant metabolic and defense response changes when the maintenance temperature fluctuates within the physiological range and that the degree of pathogenicity of the bacterial diet can further alter the worm transcriptome.


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