Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Comprehensive transcriptional landscape of porcine cardiac and skeletal muscles reveals differences of aging

Jianning Chen, Qin Zou, Daojun Lv, Yingying Wei, Muhammad Ali Raza, Yan Chen, Peilin Li, Xiaoyu Xi, Huaming Xu, Anxiang Wen, Li Zhu, Guoqing Tang, Mingzhou Li, Anan Jiang, Yihui Liu, Yuhua Fu, Yanzhi Jiang _ and Xuewei Li

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Oncotarget. 2018; 9:1524-1541. https://doi.org/10.18632/oncotarget.23290

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Abstract

Jianning Chen1,*, Qin Zou1,*, Daojun Lv2,*, Yingying Wei1, Muhammad Ali Raza3, Yan Chen1, Peilin Li2, Xiaoyu Xi1, Huaming Xu1, Anxiang Wen1, Li Zhu4, Guoqing Tang4, Mingzhou Li4, Anan Jiang4, Yihui Liu5, Yuhua Fu6, Yanzhi Jiang1 and Xuewei Li4

1Department of Zoology, College of Life Science, Sichuan Agricultural University, Ya'an, Sichuan 625014, China

2Sichuan Weimu Modern Agricultural Science and Technology Co., Ltd., Chengdu, Sichuan 611130, China

3Department of Crop Cultivation and Farming System, College of Agronomy, Sichuan Agricultural University, Chengdu, Sichuan 611130, China

4Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China

5Sichuan Animal Husbandry Station, Chengdu, Sichuan 610041, China

6School of Computer Science and Technology, Wuhan University of Technology, Wuhan, Hubei 430070, China

*These authors contributed equally to this work

Correspondence to:

Yanzhi Jiang, email: jiangyz04@163.com, xuewei.li@sicau.edu.cn

Keywords: aging; cardiac muscle; pig; skeletal muscle; transcriptome

Received: November 26, 2017     Accepted: December 08, 2017     Published: December 15, 2017

ABSTRACT

Aging significantly affects the cardiac muscle (CM) and skeletal muscles (SM). Since the aging process of CM and SM may be different, high throughput RNA sequencing was performed using CM and SM in different age conditions to evaluate the expression profiles of messenger RNA (mRNA), long non-coding RNA (lncRNA), micro RNA (miRNA), and circular (circRNA). Several mRNAs, lncRNAs, and miRNAs were highly expressed and consistently appeared in both ages in one of the two muscle tissues. Gene ontology (GO) annotation described that these genes were required for maintaining normal biological functions of CM and SM tissues. Furthermore, 26 mRNAs, 4 lncRNAs, 22 miRNAs, and 26 circRNAs were differentially expressed during cardiac muscle aging. Moreover, 81 mRNAs, 5 lncRNAs, 79 miRNAs, and 62 circRNAs were differentially expressed during aging of skeletal muscle. When comparing the expression profiles of CM and SM during aging, the senescence process in CM and SM was found to be fundamentally different. In addition, we assessed multi-group cooperative control relationships and constructed circRNA-miRNA-mRNA co-expression networks in muscular aging. In conclusion, our findings will contribute to the understanding of muscular aging and provide a foundation for future studies on the molecular mechanisms underlying muscular aging.


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