A central role for TRPS1 in the control of cell cycle and cancer development
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Lele Wu1,*, Yuzhi Wang1,*, Yan Liu1, Shiyi Yu1, Hao Xie1, Xingjuan Shi1, Sheng Qin2, Fei Ma2, Tuan Zea Tan3, Jean Paul Thiery3,4,5 and Liming Chen1
1 The Key Laboratory of Developmental Genes and Human Disease, Ministry of Education, Institute of Life Science, Southeast University, Nanjing, PR China
2 Laboratory for Comparative Genomics and Bioinformatics & Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Science, Nanjing Normal University, Nanjing, China
3 Cancer Science Institute, National University of Singapore, 14 Medical Drive, Singapore
4 Institute of Molecular and Cell Biology, A*STAR, 61 Biopolis Drive, Singapore
5 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore
* These authors contributed equally to this work
Liming Chen, email:
Keywords: Cancer development, Cell cycle control, HDAC, Histone acetylation, TRPS1
Received: April 05, 2014 Accepted: July 31, 2014 Published: July 31, 2014
The eukaryotic cell cycle is controlled by a complex regulatory network, which is still poorly understood. Here we demonstrate that TRPS1, an atypical GATA factor, modulates cell proliferation and controls cell cycle progression. Silencing TRPS1 had a differential effect on the expression of nine key cell cycle-related genes. Eight of these genes are known to be involved in the regulation of the G2 phase and the G2/M transition of the cell cycle. Using cell synchronization studies, we confirmed that TRPS1 plays an important role in the control of cells in these phases of the cell cycle. We also show that silencing TRPS1 controls the expression of 53BP1, but not TP53. TRPS1 silencing also decreases the expression of two histone deacetylases, HDAC2 and HDAC4, as well as the overall HDAC activity in the cells, and leads to the subsequent increase in the acetylation of histone4 K16 but not of histone3 K9 or K18. Finally, we demonstrate that TRPS1 expression is elevated in luminal breast cancer cells and luminal breast cancer tissues as compared with other breast cancer subtypes. Overall, our study proposes that TRPS1 acts as a central hub in the control of cell cycle and proliferation during cancer development.
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