Recombinant protein of  Haemonchus contortus  small GTPase ADP-ribosylation factor 1 (HcARF1) modulate the cell mediated immune response  in vitro

Javaid Ali Gadahi; Muhammad Ehsan; Shuai Wang; Zhenchao Zhang; Ruofeng Yan; Xiaokai Song; Lixin Xu; Xiangrui Li

doi:10.18632/oncotarget.22662

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Research Papers: Immunology:

Recombinant protein of Haemonchus contortus small GTPase ADP-ribosylation factor 1 (HcARF1) modulate the cell mediated immune response in vitro

Javaid Ali Gadahi, Muhammad Ehsan, Shuai Wang, Zhenchao Zhang, Ruofeng Yan, Xiaokai Song, Lixin Xu and Xiangrui Li _

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Oncotarget. 2017; 8:112211-112221. https://doi.org/10.18632/oncotarget.22662

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Abstract

Javaid Ali Gadahi1,2, Muhammad Ehsan1, Shuai Wang1, Zhenchao Zhang1, Ruofeng Yan1, Xiaokai Song1, Lixin Xu1 and Xiangrui Li1

1 College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, PR China

2 Department of Veterinary Parasitology, Sindh Agriculture University, Tando Jam, Pakistan

Correspondence to:

Xiangrui Li, email:

Keywords: H. contortus; ADP-ribosylation factor; PBMCs; cytokines; proliferation

Received: February 23, 2017 Accepted: November 03, 2017 Published: November 26, 2017

Abstract

ADP-ribosylation factors (ARFs) are members of the Ras-related small GTPase family involved in the vesicular trafficking regulation. Immunomodulatory effects of these proteinson host cell arenot being addressed yet. H. contortus small GTPase ADP-ribosylation 1 gene (HcARF1) was cloned and recombinant protein of HcARF1 (rHcARF1) was successfully expressed in Escherichia coli. Binding activity of rHcARF1 to goat PBMCs was confirmed by immunofluorescence assay (IFA) and its immunomudulatory effects on cytokine secretion, cell proliferation, cell migration and nitric oxide production (NO) were observed by co-incubation of rHcARF1. IFA results revealed that rHcARF1 could bind to the PBMCs. The interaction of rHcARF1 modulated the cytokine production, the production of IL-4, IL-10 and IL-17 was increased in a dose dependent manner, however, the IFN-γ production was significantly decreased. Cell migration and NO production were significantly increased by rHcARF1, whereas, rHcARF1 treatment significantly suppressed the proliferation of the PBMC in a dose dependent manner. Our findings showed that the rHcARF1 play important roles on the goat PBMCs.

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Research Papers: Immunology:

Recombinant protein of Haemonchus contortus small GTPase ADP-ribosylation factor 1 (HcARF1) modulate the cell mediated immune response in vitro

Abstract