The value of lncRNA FENDRR and FOXF1 as a prognostic factor for survival of lung adenocarcinoma
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Antonio Herrera-Merchan1,2,*, Marta Cuadros1,3,*, Maria Isabel Rodriguez1,2, Sandra Rodriguez4, Raul Torres4, Marcos Estecio5, Isabel F. Coira1,2, Claudia Loidi6, Monica Saiz6, Pedro Carmona-Saez1 and Pedro P. Medina1,2
1Centre for Genomics and Oncological Research, PTS Granada, Centro Pfizer - Universidad de Granada - Junta de Andalucía de Genómica e Investigación Oncológica (GENYO), Granada, Spain
2Department of Biochemistry and Molecular Biology I, University of Granada, Granada, Spain
3Department of Biochemistry and Molecular Biology III e Immunology, University of Granada, Granada, Spain
4Molecular Cytogenetics Group, Human Cancer Genetics Program, Spanish National Cancer Research Centre-CNIO, Madrid, Spain
5Department of Epigenetics and Molecular Carcinogenesis, UT MD Anderson Cancer Center, Houston, TX, USA
6Pathological Anatomy, Universitary Hospital Cruces, University of Pais Vasco, Spain
*These authors have contributed equally to this work
Pedro P. Medina, email: email@example.com
Keywords: lncRNA; FENDRR; FOXF1; lung cancer; methylation
Received: June 09, 2017 Accepted: October 02, 2017 Published: October 27, 2017
It is increasingly evident that non-coding RNAs play a significant role in tumour development. However, we still have a limited knowledge of the clinical significance of long non-coding RNAs (lncRNAs) in lung cancer. The FENDRR is a long coding RNA (also named FOXF1-AS1) located in the vicinity of the protein-coding gene FOXF1 at 16q24.1 chromosomal region. The present study aimed to define the clinic pathological significance of the long-non-coding RNA FENDRR in lung adenocarcinomas. FENDRR expression measured by quantitative PCR was found significantly downregulated (p<0.001) in lung adenocarcinoma samples in comparison with their normal adjacent tissues (n=70). RNA in situ hybridization (RNA-FISH) corroborated independently the down-regulation of FENDRR. Interestingly, the expression of FENDRR correlated positively (p<0.001) with the expression of its protein-coding neighbor gene FOXF1. Additionally, FOXF1 expression was also found downregulated in adenocarcinomas compared to normal samples (p<0.001) and its expression was significantly correlated with overall survival alone (p=0.003) or in combination with FENDRR expression (p=0.01). In conclusion, our data support that FENDRR and FOXF1 expression is decreased in lung adenocarcinoma and should be considered as new potential diagnostic/prognosis biomarkers.
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