Oncotarget

Research Papers:

Plasmatic miR-210, miR-221 and miR-1233 profile: potential liquid biopsies candidates for renal cell carcinoma

Francisca Dias, Ana Luísa Teixeira _, Marta Ferreira, Bárbara Adem, Nuno Bastos, Joana Vieira, Mara Fernandes, Maria Inês Sequeira, Joaquina Maurício, Francisco Lobo, António Morais, Jorge Oliveira, Klaas Kok and Rui Medeiros

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Oncotarget. 2017; 8:103315-103326. https://doi.org/10.18632/oncotarget.21733

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Abstract

Francisca Dias1,2,3, Ana Luísa Teixeira1,2, Marta Ferreira4, Bárbara Adem1,5, Nuno Bastos1,5, Joana Vieira6, Mara Fernandes1,2,5, Maria Inês Sequeira4, Joaquina Maurício4, Francisco Lobo7, António Morais7, Jorge Oliveira7, Klaas Kok8 and Rui Medeiros1,2,5,9

1Molecular Oncology and Viral Pathology Group, IPO-Porto Research Center (CI-IPOP), Portuguese Oncology Institute of Porto (IPO-Porto), Porto, Portugal

2Research Department, LPCC- Portuguese League Against Cancer (NRNorte), Porto, Portugal

3ICBAS, Abel Salazar Institute for the Biomedical Sciences, University of Porto, Porto, Portugal

4Medical Oncology Department of the Portuguese Oncology Institute of Porto (IPO-Porto), Porto, Portugal

5FMUP, Faculty of Medicine, University of Porto, Porto, Portugal

6Genetics Department of the Portuguese Oncology Institute of Porto (IPO-Porto), Porto, Portugal

7Urology Department of the Portuguese Oncology Institute of Porto (IPO-Porto), Porto, Portugal

8Department of Genetics, University Medical Center, Groningen, The Netherlands

9CEBIMED, Faculty of Health Sciences, Fernando Pessoa University, Porto, Portugal

Correspondence to:

Ana Luísa Teixeira, email: ana.luisa.teixeira@ipoporto.min-saude.pt

Rui Medeiros, email: ruimedei@ipoporto.min-saude.pt

Keywords: circulating miRNAs, renal cell carcinoma, prognostic biomarkers

Received: January 12, 2017     Accepted: September 25, 2017     Published: October 11, 2017

ABSTRACT

Renal cell carcinoma (RCC) represents a challenge for clinicians since the nonexistence of screening and monitoring tests contributes to the fact that one-third of patients are diagnosed with metastatic disease and 20–40% of the remaining patients will also develop metastasis. Modern medicine is now trying to establish circulating biomolecules as the gold standard of biomarkers. Among the molecules that can be released from tumor cells we can find microRNAs. The aim of this study was to evaluate the applicability of cancer-related miR-210, miR-218, miR-221 and miR-1233 as prognostic biomarkers for RCC. Patients with higher levels of miR-210, miR-221 and miR-1233 presented a higher risk of specific death by RCC and a lower cancer-specific survival. The addition of miR-210, miR-221 and miR-1233 plasma levels information improved the capacity to predict death by cancer in 8, 4% when compared to the current variables used by clinicians. We also verified that hypoxia stimulates the release of miR-210 and miR-1233 from HKC-8, RCC-FG2 and 786-O cell lines. These results support the addition of circulating microRNAs as prognostic biomarkers for RCC.


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