Exploring the model of PC12 apoptosis induced by OGSD/R through  in vitro  experiments

Yanqing Sun; Wei Zhu; Shengyuan Zhou; Zhiwei Wang; Xiongsheng Chen; Lianshun Jia

doi:10.18632/oncotarget.21623

Oncotarget

Oncotarget (a primarily oncology-focused, peer-reviewed, open access journal) aims to maximize research impact through insightful peer-review; eliminate borders between specialties by linking different fields of oncology, cancer research and biomedical sciences; and foster application of basic and clinical science.

Its scope is unique. The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. The term was introduced in the inaugural Editorial, Introducing Oncotarget.

As of January 1, 2022, Oncotarget has shifted to a continuous publishing model. Papers will now be published continuously within yearly volumes in their final and complete form and then quickly released to Pubmed.

Subscribe to receive alerts once a paper has been published by Oncotarget.

Impact Journals, LLC is the publisher of Oncotarget: www.impactjournals.com.

Impact Journals is a member of the Wellcome Trust List of Compliant Publishers.

Impact Journals is a member of the Society for Scholarly Publishing.

On December 23, 2022, Oncotarget server experienced a DDoS attack. As a result, Oncotarget site was inaccessible for a few hours. Oncotarget team swiftly dealt with the situation and took it under control. This malicious action will be reported to the FBI.

Research Papers:

Exploring the model of PC12 apoptosis induced by OGSD/R through in vitro experiments

Yanqing Sun, Wei Zhu, Shengyuan Zhou, Zhiwei Wang, Xiongsheng Chen _ and Lianshun Jia

PDF | HTML | How to cite

Oncotarget. 2017; 8:90176-90184. https://doi.org/10.18632/oncotarget.21623

Metrics: PDF 1374 views | HTML 1797 views | ?

Abstract

Yanqing Sun1,*, Wei Zhu1,*, Shengyuan Zhou1, Zhiwei Wang1, Xiongsheng Chen1 and Lianshun Jia1

1Department of Spine, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China

*These authors are considereds as co-first authors

Correspondence to:

Xiongsheng Chen, email: [email protected]

Lianshun Jia, email: [email protected]

Keywords: oxygen-glucose-serum deprivation/restoration, PC12, apoptosis, ischemia reperfusion

Received: March 01, 2017 Accepted: August 06, 2017 Published: October 04, 2017

ABSTRACT

Aims: To explicit cell apoptosis trend in PC12 oxygen-glucose-serum deprivation/restoration (OGSD/R) model and provide experimental bases for neural cell simulation in ischemia reperfusion injury in vitro.

Methods: OGSD/R model was constructed using the passage PC12 cells in vitro. The profile of cell apoptosis was estimated by DAPI staining, Annexin V-FITC and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay, as well as the levels of apoptosis-related proteins, including procaspase-3 and caspase-12.

Results: PC12 apoptosis was induced by OGSD and aggravated after restoration. CCK8 assay indicated that cell activity reached minimum after 1h of oxygen-glucose-serum restoration (OGR). DAPI staining suggested that apoptosis was the most serious after 1h of OGR, causing apoptotic cell nucleus pyknosis, particle spot formation, and fracture of cells with serious apoptosis forming pieces, and nucleus disintegration. The percentage of apoptotic cells exhibited increased trend after restoration, and reached the highest at 1h of OGR. Moreover, the expression of procaspase-3 and caspase-12 were extremely enhanced after OGD, especially 1h after OGR.

Conclusions: PC12 apoptosis is induced by OGSD and aggravated after restoration. The apoptosis of PC12 reaches the highest at 1h after OGR, which may provide experimental bases for spinal cord ischemia reperfusion injury treatment.

All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 4.0 License.
PII: 21623

Publication Alerts

Research Papers:

Exploring the model of PC12 apoptosis induced by OGSD/R through in vitro experiments

Abstract