Oncotarget

Research Papers: Gerotarget (Focus on Aging):

PINK1 suppresses alpha-synuclein-induced neuronal injury: a novel mechanism in protein phosphatase 2A activation

Weiwei Yang, Xue Wang, Jia Liu, Chunli Duan, Ge Gao, Lingling Lu, Shun Yu and Hui Yang _

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Oncotarget. 2018; 9:37-53. https://doi.org/10.18632/oncotarget.21554

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Abstract

Weiwei Yang1,2,*, Xue Wang1,*, Jia Liu1,*, Chunli Duan1, Ge Gao1, Lingling Lu1, Shun Yu2 and Hui Yang1

1 Department of Neurobiology, Center for Parkinson’s Disease, Beijing Institute for Brain Disorders, Key Laboratory for Neurodegenerative Diseases of the Ministry of Education, Capital Medical University, Beijing, China

2 Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing, China

* These authors have contributed equally to this work

Correspondence to:

Hui Yang, email:

Shun Yu, email:

Keywords: PINK1, alpha-synuclein (α-Syn), Parkinson’s disease (PD), protein phosphatase 2A (PP2A), Src kinase, Gerotarget

Received: August 12, 2017 Accepted: September 22, 2017 Published: October 06, 2017

Abstract

Alpha-synuclein (α-Syn) and phosphatase and tensin homolog deleted on chromosome ten (PTEN)-induced putative kinase (PINK) 1 are proteins found in Lewy bodies, which are a pathological hallmark of Parkinson’s disease (PD). PINK1 overexpression suppresses α-Syn-induced phenotypes and increases lifespan and health in an animal model of PD. It has been suggested that the two proteins regulate protein phosphatase (PP) 2A activity, but the underlying mechanisms and neuroprotective action of PP2A against PD-associated pathology are unknown. We found that α-Syn overexpression in SK-N-SH neuroblastoma cells and primary cortical neurons caused mitochondrial dysfunction and cell injury via phosphorylation of PP2A at Tyr307 and inhibition of its activity. Concomitant overexpression of PINK1 reversed this effect and restored the activity. The level of phospho-activated Src was increased in cells overexpressing α-Syn, which was reversed by co-expressing PINK1, suggesting that the latter suppressed α-Syn-induced PP2A inactivation by inhibiting Src activity. Calmodulin/Src complex formation was also enhanced in α-Syn-overexpressing cells, which was reversed by co-expression of PINK1 as a result of reduced mitochondrial Ca2+ releasing. Interestingly, the protective effects of PINK1 in α-Syn induced models were abolished by treatment with the PP2A inhibitor okadaic acid, indicating that PP2A is a target of PINK1. These findings indicate that PINK1 protects against α-Syn-induced neurodegeneration by promoting the dissociation of the calmodulin/Src complex and inhibiting Src, thereby enhancing PP2A activity. This was supported by the observation that PP2A activity was decreased in PD patients, which was negatively correlated with Hoehn and Yahr scores. Our results provide novel insight into the mechanisms underlying neurodegeneration in PD as well as possible avenues for therapeutic intervention in the treatment of this disease.


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