Oncotarget

Research Papers:

Differently expressed long noncoding RNAs and mRNAs in TK6 cells exposed to low dose hydroquinone

Shaoyun Chen, Hairong Liang, Gonghua Hu, Hui Yang, Kairu Zhou, Longmei Xu, Jiaxian Liu, Bei Lai, Li Song, Hao Luo, Jianming Peng, Zhidong Liu, Yongmei Xiao, Wen Chen and Huanwen Tang _

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Oncotarget. 2017; 8:95554-95567. https://doi.org/10.18632/oncotarget.21481

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Abstract

Shaoyun Chen1,*, Hairong Liang1,*, Gonghua Hu2,*, Hui Yang1, Kairu Zhou1, Longmei Xu1, Jiaxian Liu1, Bei Lai1, Li Song1, Hao Luo1, Jianming Peng3, Zhidong Liu3, Yongmei Xiao4, Wen Chen4 and Huanwen Tang1

1Department of Environmental and Occupational Health, Dongguan Key Laboratory of Environmental Medicine, School of Public Health, Guangdong Medical University, Dongguan, 523808, China

2Department of Preventive Medicine, Gannan Medical University, Ganzhou, 341000, China

3Huizhou Prevention and Treatment Centre for Occupational Disease, Huizhou, 516000, China

4Guangzhou Key Laboratory of Environmental Pollution and Health Risk Assessment, Department of Toxicology, School of Public Health, Sun Yat-sen University, Guangzhou, 510080, China

*These authors contributed equally to this work

Correspondence to:

Huanwen Tang, email: thw@gdmu.edu.cn

Keywords: long noncoding RNA (lncRNA), hydroquinone, high-throughput sequencing, expression profiles, leukemia

Received: August 01, 2017     Accepted: September 20, 2017     Published: October 04, 2017

ABSTRACT

Previous studies have shown that long noncoding RNAs (lncRNAs) were related to human carcinogenesis and might be designated as diagnosis and prognosis biomarkers. Hydroquinone (HQ), as one of the metabolites of benzene, was closely relevant to occupational benzene poisoning and occupational leukemia. Using high-throughput sequencing technology, we investigated differences in lncRNA and mRNA expression profiles between experimental group (HQ 20 μmol/L) and control group (PBS). Compared to control group, a total of 65 lncRNAs and 186 mRNAs were previously identified to be aberrantly expressed more than two fold change in experimental group. To validate the sequencing results, we selected 10 lncRNAs and 10 mRNAs for quantitative real-time PCR (qRT-PCR). Through GO annotation and KEGG pathway analysis, we obtained 3 mainly signaling pathways, including P53 signaling pathway, which plays an important role in tumorigenesis and progression. After that, 25 lncRNAs and 32 mRNAs formed the lncRNA-mRNA co-expression network were implemented to play biological functions of the dysregulated lncRNAs transcripts by regulating gene expression. The lncRNAs target genes prediction provided a new idea for the study of lncRNAs. Finally, we have another important discovery, which is screened out 11 new lncRNAs without annotated. All these results uncovered that lncRNA and mRNA expression profiles in TK6 cells exposed to low dose HQ were different from control group, helping to further study the toxicity mechanisms of HQ and providing a new direction for the therapy of leukemia.


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