Oncotarget

Research Papers:

Molecular characteristics and serodiagnostic potential of chitinase-like protein from Sarcoptes scabiei

Ran He, Nengxing Shen, Haojie Zhang, Yongjun Ren, Manli He, Jing Xu, Cheng Guo, Yue Xie, Xiaobin Gu, Weimin Lai, Xuerong Peng and Guangyou Yang _

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Oncotarget. 2017; 8:83995-84005. https://doi.org/10.18632/oncotarget.21056

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Abstract

Ran He1,*, Nengxing Shen1,*, Haojie Zhang1,*, Yongjun Ren2, Manli He1, Jing Xu1, Cheng Guo1, Yue Xie1, Xiaobin Gu1, Weimin Lai1, Xuerong Peng3 and Guangyou Yang1

1Department of Parasitology, College of Veterinary Medicine, Sichuan Agricultural University, Wenjiang, China

2Sichuan Animal Sciences Academy, Sichuan Chengdu, China

3Department of Chemistry, College of Life and Basic Science, Sichuan Agricultural University, Wenjiang, China

*These authors contributed equally to this work

Correspondence to:

Guangyou Yang, email: [email protected]

Keywords: chitinase-like protein, Sarcoptes scabiei, enzyme-linked immunosorbent assay, immunolocalization, intradermal skin test

Received: May 23, 2017     Accepted: September 03, 2017     Published: September 19, 2017

ABSTRACT

Scabies, caused by the mite Sarcoptes scabiei, is an allergic skin disease that affects millions of people and other mammals worldwide. This highly contagious parasitic disease is among the top 50 epidemic disease and is regarded as a neglected tropical disease. Diagnosis of scabies is difficult in the early stage, and the pathogenesis of scabies is not currently clear. Here, we expressed, identified and located the chitinase-like protein of S. scabiei (SsCLP), and evaluated its potential as an early-stage diagnostic antigen for rabbit scabies. Indirect ELISA using recombinant SsCLP (rSsCLP) exhibited diagnostic sensitivity of 94.4% (17/18) and specificity of 86.7% (26/30). Early diagnostic test after artificial infection of rabbits with S. scabiei for 1 week showed a positive detection rate of 96.7% (29/30). Immunolocalization assays showed that fluorescence signals were localized on the surface of mites and, in infected rabbits, were observed in keratinized skin and embedded mites. Intradermal skin tests of rabbits by injecting rSsCLP showed a wheal, flare and erythema reaction. These results suggest that S. scabiei chitinase-like protein is conducive to host invasion, participates in inducing the allergic response of the host, and is an effective antigen for the diagnosis of S. scabiei.


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