Oncotarget

Research Papers:

Effects of trimetazidine on periprocedural microRNA-21 expression by CD4+ T lymphocytes in patients with unstable angina pectoris

Qiang Su, Lang Li _, Jinmin Zhao, Yuhan Sun and Huafeng Yang

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Oncotarget. 2017; 8:104992-104999. https://doi.org/10.18632/oncotarget.20975

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Abstract

Qiang Su1,3, Lang Li1, Jinmin Zhao2,3, Yuhan Sun1 and Huafeng Yang1

1Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

2Department of Trauma Orthopedic and Hand Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning 530021, China

3Guangxi Key Laboratory of Regenerative Medicine, Guangxi Medical University, Guangxi 530021, China

Correspondence to:

Lang Li, email: drlangli@yeah.net

Jinmin Zhao, email: drzhaojinmin@163.com

Keywords: unstable angina pectoris, trimetazidine, CD4+ T lymphocyte, microRNA-21, PDCD4

Received: April 05, 2017    Accepted: August 17, 2017    Published: September 18, 2017

ABSTRACT

Objective: Post-percutaneous coronary intervention (PCI) myocardial injury is related to the CD4+ T lymphocyte-mediated inflammatory response. microRNA-21 expression is associated with CD4+ T lymphocyte activation. The pre-PCI use of trimetazidine prevents periprocedural myocardial injury and reduces inflammatory cytokine levels. This study aimed to assess the effects of trimetazidine on periprocedural microRNA-21 expression by CD4+ T lymphocytes in patients with unstable angina pectoris.

Methods: A total of 252 patients with unstable angina pectoris were randomized to the trimetazidine (60 mg/d, administered 3 days before PCI, n=128) and control (no trimetazidine, n=124) groups. Serum CK-MB, cTnI, and hs-CRP levels were tested pre-PCI and 16-24 h post-PCI. Peripheral blood CD4+ T lymphocytes were isolated by magnetic activated cell sorting. Quantitative polymerase chain reaction was used to assess microRNA-21 and PDCD4 mRNA expression levels in CD4+ T lymphocytes, and western blot was used to evaluate PDCD4 protein expression. Enzyme-linked immunosorbent assay was used to assess serum TNF-α and IL-10 levels.

Results: Compared with the control group, the trimetazidine group had a lower frequency of patients with post-PCI serum CK-MB and cTnI levels higher than normal values; the trimetazidine group had also significantly lower serum hs-CRP and TNF-α levels, and higher IL-10 levels post-PCI. Finally, the trimetazidine group had significantly lower PDCD4 expression and higher microRNA-21 levels in CD4+ T lymphocytes post-PCI.

Conclusions: Trimetazidine reduces the incidence of periprocedural myocardial injury, possibly by increasing microRNA-21 levels in CD4+ T lymphocytes and inhibiting PDCD4-mediated inflammatory response.


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