Oncotarget

Research Papers: Gerotarget (Focus on Aging):

Caloric restriction extends yeast chronological lifespan via a mechanism linking cellular aging to cell cycle regulation, maintenance of a quiescent state, entry into a non-quiescent state and survival in the non-quiescent state

Anna Leonov, Rachel Feldman, Amanda Piano, Anthony Arlia-Ciommo, Vicky Lutchman, Masoumeh Ahmadi, Sarah Elsaser, Hana Fakim, Mahdi Heshmati-Moghaddam, Asimah Hussain, Sandra Orfali, Harshvardhan Rajen, Negar Roofigari-Esfahani, Leana Rosanelli and Vladimir I. Titorenko _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:69328-69350. https://doi.org/10.18632/oncotarget.20614

Metrics: PDF 568 views  |   HTML 1238 views  |   ?  


Abstract

Anna Leonov1, Rachel Feldman1, Amanda Piano1, Anthony Arlia-Ciommo1, Vicky Lutchman1, Masoumeh Ahmadi1, Sarah Elsaser1, Hana Fakim1, Mahdi Heshmati-Moghaddam1, Asimah Hussain1, Sandra Orfali1, Harshvardhan Rajen1, Negar Roofigari-Esfahani1, Leana Rosanelli1 and Vladimir I. Titorenko1

1 Department of Biology, Concordia University, Montreal, Quebec, Canada

Correspondence to:

Vladimir I. Titorenko, email:

Keywords: yeast, cellular aging, cell cycle, cell differentiation, cell quiescence, Gerotarget 

Received: June 12, 2017 Accepted: August 14, 2017 Published: September 01, 2017

Abstract

A yeast culture grown in a nutrient-rich medium initially containing 2% glucose is not limited in calorie supply. When yeast cells cultured in this medium consume glucose, they undergo cell cycle arrest at a checkpoint in late G1 and differentiate into quiescent and non-quiescent cell populations. Studies of such differentiation have provided insights into mechanisms of yeast chronological aging under conditions of excessive calorie intake. Caloric restriction is an aging-delaying dietary intervention. Here, we assessed how caloric restriction influences the differentiation of chronologically aging yeast cultures into quiescent and non-quiescent cells, and how it affects their properties. We found that caloric restriction extends yeast chronological lifespan via a mechanism linking cellular aging to cell cycle regulation, maintenance of quiescence, entry into a non-quiescent state and survival in this state. Our findings suggest that caloric restriction delays yeast chronological aging by causing specific changes in the following: 1) a checkpoint in G1 for cell cycle arrest and entry into a quiescent state; 2) a growth phase in which high-density quiescent cells are committed to become low-density quiescent cells; 3) the differentiation of low-density quiescent cells into low-density non-quiescent cells; and 4) the conversion of high-density quiescent cells into high-density non-quiescent cells.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 20614