Oncotarget

Research Papers:

Wnt/β-catenin and Hedgehog pathways are involved in the inflammatory effect of Interleukin 18 on rat chondrocytes

Jiapeng Bao, Chiyuan Ma, Jisheng Ran, Yan Xiong, Shigui Yan and Lidong Wu _

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Oncotarget. 2017; 8:109962-109972. https://doi.org/10.18632/oncotarget.20584

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Abstract

Jiapeng Bao1, Chiyuan Ma1, Jisheng Ran1, Yan Xiong1, Shigui Yan1 and Lidong Wu1

1Department of Orthopedics Surgery, The 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310000 China

Correspondence to:

Lidong Wu, email: wulidong@zju.edu.cn

Shigui Yan, email: zrjwsj@zju.edu.cn

Keywords: Interleukin 18; chondrocyte; osteoarthritis; Wnt/β-catenin; Hedgehog

Received: April 23, 2017    Accepted: August 08, 2017    Published: August 24, 2017

ABSTRACT

To investigate the inflammatory effect of Interleukin 18 (IL-18) on rat chondrocytes and the involvement of Wnt/β-catenin and Hedgehog pathways, the mRNA and protein level of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and chondrocyte-specific proteins (Collagen II and aggrecan) were evaluated by qRT-PCR and Western blot, and key protein level of Wnt/β-catenin and Hedgehog pathways including β-catenin, GSK-3β, DKK-1, IHH, SHH, and Gli-2 were evaluated by Western blot. Dickkopf-1 (DKK-1) and Cyclopamine were used as antagonist of Wnt/β-catenin and Hedgehog pathways to perform pathway inhibition tests. In addition, location and expression of β-catenin, GSK-3β, Gli-2 and Smo were assessed by Immunofluorescence microscopy. The results showed up-regulation of matix-degrading enzymes (MMP-2, 3, 9,13 and aggrecanses) and down-regulation of chondrocyte-specific proteins (Collagen II and aggrecan) at both mRNA and protein level and activation of Wnt/β-catenin and Hedgehog pathways in the inflammatory reaction on rat chondrocytes caused by IL-18 treatment was observed. As conclusion, Wnt/β-catenin and Hedgehog pathways are involved in the inflammatory effect of IL-18 in vitro.


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