Oncotarget

Research Papers:

Development and cytotoxic response of two proliferative MDA-MB-231 and non-proliferative SUM1315 three-dimensional cell culture models of triple-negative basal-like breast cancer cell lines

Dubois Clémence, Dufour Robin, Daumar Pierre, Aubel Corinne, Szczepaniak Claire, Blavignac Christelle, Mounetou Emmanuelle, Penault-Llorca Frédérique and Mahchid Bamdad _

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Oncotarget. 2017; 8:95316-95331. https://doi.org/10.18632/oncotarget.20517

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Abstract

Dubois Clémence1,2, Dufour Robin1,2, Daumar Pierre1, Aubel Corinne3, Szczepaniak Claire4, Blavignac Christelle4, Mounetou Emmanuelle1,*, Penault-Llorca Frédérique2,* and Mahchid Bamdad1,5

1Université Clermont Auvergne, Institut Universitaire de Technologie, INSERM, U1240, Imagerie Moléculaire et Stratégies Théranostiques, F-63000 Clermont Ferrand, France

2Université Clermont Auvergne, Centre Jean Perrin, INSERM, U1240, Imagerie Moléculaire et Stratégies Théranostiques, F-63000 Clermont Ferrand, France

3Université Clermont Auvergne, Faculté de médecine, INSERM, U1240, Imagerie Moléculaire et Stratégies Théranostiques, F-63000 Clermont Ferrand, France

4Université Clermont Auvergne, Faculté de Médecine, Centre Imagerie Cellulaire Santé, F-63000 Clermont-Ferrand, France

5Current address: Institut Universitaire de Technologie de Clermont-Ferrand – Université Clermont Auvergne, Département Génie Biologique, Ensemble Universitaire des Cézeaux, CS 30086- 63172 AUBIERE CEDEX, France

*These authors have contributed equally to this work

Correspondence to:

Mahchid Bamdad, email: mahchid.bamdad@uca.fr

Keywords: triple negative basal-like breast cancer cell lines; 3D cell culture; liquid overlay technique; cytotoxic/cytostatic drug activity

Received: June 06, 2017    Accepted: July 31, 2017    Published: August 24, 2017

ABSTRACT

Triple-Negative Basal-Like tumors, representing 15 to 20% of breast cancers, are very aggressive and with poor prognosis. Targeted therapies have been developed extensively in preclinical and clinical studies to open the way for new treatment strategies. The present study has focused on developing 3D cell cultures from SUM1315 and MDA-MB-231, two triple-negative basal-like (TNBL) breast cancer cell lines, using the liquid overlay technique. Extracellular matrix concentration, cell density, proliferation, cell viability, topology and ultrastructure parameters were determined. The results showed that for both cell lines, the best conditioning regimen for compact and homogeneous spheroid formation was to use 1000 cells per well and 2% Geltrex®. This conditioning regimen highlighted two 3D cell models: non-proliferative SUM1315 spheroids and proliferative MDA-MB-231 spheroids. In both cell lines, the comparison of 2D vs 3D cell culture viability in the presence of increasing concentrations of chemotherapeutic agents i.e. cisplatin, docetaxel and epirubicin, showed that spheroids were clearly less sensitive than monolayer cell cultures. Moreover, a proliferative or non-proliferative 3D cell line property would enable determination of cytotoxic and/or cytostatic drug activity. 3D cell culture could be an excellent tool in addition to the arsenal of techniques currently used in preclinical studies.


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