Immunology repertoire study of pulmonary sarcoidosis T cells in CD4+, CD8+ PBMC and tissue

Yingyun Fu; Yazhen Li; Lan Xu; Shengguo Liu; Minlian Wang; Lu Xiao; Song Liu; Yong Dai

doi:10.18632/oncotarget.20085

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Research Papers: Immunology:

Immunology repertoire study of pulmonary sarcoidosis T cells in CD4+, CD8+ PBMC and tissue

Yingyun Fu _, Yazhen Li, Lan Xu, Shengguo Liu, Minlian Wang, Lu Xiao, Song Liu and Yong Dai

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Oncotarget. 2017; 8:89515-89526. https://doi.org/10.18632/oncotarget.20085

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Abstract

Yingyun Fu1,3,*, Yazhen Li1,*, Lan Xu1, Shengguo Liu1, Minlian Wang3, Lu Xiao1, Song Liu2 and Yong Dai2

1 Department of Respiratory and Critical Care Medicine, The Second Medical College of Jinan University, Shenzhen People’s Hospital, Luohu District, Shenzhen, China

2 Clinical Medical Research Center, The Second Clinical Medical College of Jinan University, Shenzhen People’s Hospital, Luohu District, Shenzhen, China

3 Shenzhen Key Laboratory of Respiratoty Disease, Shenzhen, China

* These authors have contributed equally to this work

Correspondence to:

Yingyun Fu, email:

Keywords: sarcoidosis, immune system, CDR3, TCR BV CDR3, PMBCs, Immunology and Microbiology Section, Immune response, Immunity

Received: April 08, 2017 Accepted: July 30, 2017 Published: August 09, 2017

Abstract

Sarcoidosis is a systemic granulomatous disorder highly related with immune response. The diversity and stability of the immune system could be measured by hypervariable complementarity-determining region 3 (CDR3) segments of the T cell receptor (TCR). Here we used a combination of multiplex PCR and next-generation sequencing to conduct a good quality analysis of the T-cell receptor BV complementarity-determining region 3 (TCR BV CDR3) gene in peripheral blood mononuclear cells (PBMCs) from 7 sarcoidosis patients and lung sarcoidosis tissue from 6 patients. The length distribution of CDR3 sequences identified a significant difference among CD4+, CD8+ and tissue samples. The analysis of Gini coefficient, Shannon entropy and HEC number showed that they all presents in sarcoidosis tissue group clones in a more skewed manner than that of in PMBCs groups. 2 nucleotide sequences and 2 amino acid sequences were shared by all samples. The comparison of TRBV, TRBJ usage and VJ combination frequency identified 2 TRBV genes, 2 TRBJ genes differentially expressed among different groups and different higher usage and lower usage of V-J combinations between each group.

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Research Papers: Immunology:

Immunology repertoire study of pulmonary sarcoidosis T cells in CD4+, CD8+ PBMC and tissue

Abstract