Research Papers:
The significance of ENAH in carcinogenesis and prognosis in gastric cancer
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Abstract
Dan-Dan Wang1,*, Qun Jin2,*, Lei-Lei Wang3, Shu-Fang Han2, Yi-Bing Chen4, Guo-Dong Sun5, Shi-Fei Sun1, Shu-Wang Sun1, Tao Wang1, Fan-Jie Liu1, Ping Wang6,7 and Bin Shi1
1Shandong Medicinal Biotechnology Centre, Key Laboratory for Rare & Uncommon Diseases of Shandong Province, Back and Neck Pain Hospital of Shandong Academy of Medical Sciences, Jinan 250062, People's Republic of China
2The General Hospital of Jinan Military Command, Jinan 250012, People's Republic of China
3Key Laboratory for Applied Microbiology of Shandong Province, Ecology Institute of Shandong Academy of Sciences, Jinan 250014, People's Republic of China
4Center of Genetic & Prenatal Diagnosis, First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, People's Republic of China
5Affiliated Hospital of Shandong Academy of Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250031, People's Republic of China
6School of Precision Instrument and Opto Electronics Engineering, Tianjin University, Tianjin 300072, People's Republic of China
7Shandong Academy of Chinese Medicine, Jinan 250014, People's Republic of China
*These authors have contributed equally to this work
Correspondence to:
Bin Shi, email: [email protected]
Ping Wang, email: [email protected]
Keywords: gastric adenocarcinoma, ENAH, expression, prognosis, function
Received: February 15, 2017 Accepted: July 12, 2017 Published: August 02, 2017
ABSTRACT
The ENAH gene, which encodes a member of the enabled/vasodilator-stimulated phosphoprotein (Ena/VASP) family of proteins, is involved in the assembly of actin filaments required for cell adhesion and motility. Recent studies show overexpressed ENAH in several cancer types, and ENAH correlates with tumor invasiveness. This study aimed to investigate the expression and function of ENAH in primary gastric adenocarcinoma, and its prognostic significance. We found significantly increased mRNA (P = 0.0283) and protein (P = 0.0301) expression of ENAH in gastric cancer tissues. ENAH expression markedly associated with tumor size (P < 0.001), T stage (P < 0.001), N stage (P = 0.001), TNM stage (P < 0.001) and prognosis (P < 0.001). Cox regression analyses revealed ENAH expression as an independent predictor of overall survival (P = 0.019). We also analyzed data of 155 gastric cancer cases from The Cancer Genome Atlas (TCGA) and found that ENAH expression significantly correlated with age (P = 0.003), T stage (P = 0.023) and prognosis (P = 0.05). Furthermore, the function of ENAH in cell proliferation, colony formation, cell migration and invasion of gastric cancer cells was analyzed in vitro. Knockdown of ENAH expression suppressed cell proliferation, colony formation, cell migration and invasion in MKN45 cells. Conversely, overexpression of ENAH promoted cell proliferation, cell migration and invasion in MGC803 cells. Our research suggests that ENAH might play promoting functions in carcinogenesis and progression of gastric cancer, and may serve as a valuable prognostic marker for primary gastric adenocarcinoma patients.
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