Oncotarget

Research Papers:

pSY153-MDR, a p12969-DIM-related mega plasmid carrying blaIMP-45 and armA, from clinical Pseudomonas putida

Min Yuan, Hai Chen, Xiong Zhu, Jiao Feng, Zhe Zhan, Defu Zhang, Xia Chen, Xiaofei Zhao, Jinxing Lu, Jianguo Xu, Dongsheng Zhou and Juan Li _

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Oncotarget. 2017; 8:68439-68447. https://doi.org/10.18632/oncotarget.19496

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Abstract

Min Yuan1, Hai Chen2, Xiong Zhu2, Jiao Feng3, Zhe Zhan3, Defu Zhang3, Xia Chen1, Xiaofei Zhao1, Jinxing Lu1, Jianguo Xu1, Dongsheng Zhou3 and Juan Li1

1State Key Laboratory of Infectious Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Disease, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China

2Department of Clinical Laboratory, People’s Hospital of Sanya, Hainan 572000, China

3State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China

Correspondence to:

Juan Li, email: lijuan@icdc.cn

Dongsheng Zhou, email: dongshengzhou1977@gmail.com

Keywords: Pseudomonas putida, multidrug resistant, IMP-45, armA, pSY153-MDR

Received: May 07, 2017    Accepted: June 30, 2017    Published: July 22, 2017

ABSTRACT

This work characterized mega plasmid pSY153-MDR, carrying blaIMP-45 and armA, from a multidrug-resistant (MDR) Pseudomonas putida isolate from the urine of a cerebral infarction patient in China. The backbone of pSY153-MDR was closely related to Pseudomonas plasmids p12969-DIM, pOZ176, pBM413, pTTS12, and pRBL16, and could not be assigned to any of the known incompatibility groups. The accessory modules of pSY153-MDR were composed of 10 individual insertion sequence elements and two different MDR regions, and differed dramatically from the above plasmids. Fifteen non-redundant resistance markers were identified to be involved in resistance to at least eight distinct classes of antibiotics. All of these resistance genes were associated with mobile elements, and were embedded within the two MDR regions. blaIMP-45 and armA coexisted in a Tn1403–Tn1548 region, which was generated from homologous recombination of Tn1403- and Tn1548-like transposons. The second copy of armA was a component of the ISCR28armA–∆ISCR28 structure, representing a novel armA vehicle. This vehicle was located within In48, which was related to In363 and In1058. Data presented here provide a deeper insight into the evolutionary history of SY153, especially in regard to how it became extensively drug-resistant.


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