Research Papers:
Diagnostic potential of serum exosomal colorectal neoplasia differentially expressed long non-coding RNA (CRNDE-p) and microRNA-217 expression in colorectal carcinoma
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Abstract
Bo Yu1,2,*, Qiong Du1,2,*, Huan Li1,2, Hong-Yue Liu1,2, Xuan Ye1,2, Bin Zhu1,2, Qing Zhai1,2 and Xin-Xiang Li1,3
1Department of Oncology, Shanghai Medical College, Fudan University, Shanghai 200032, China
2Department of Pharmacy, Fudan University Shanghai Cancer Center, Shanghai 200032, China
3Department of Colorectal Surgery, Fudan University Shanghai Cancer Center, Shanghai 200032, China
*These authors contributed equally to this work
Correspondence to:
Qing Zhai, email: [email protected]
Xin-Xiang Li, email: [email protected]
Keywords: colorectal carcinoma, exosome, CRNDE-p, miR-217
Received: March 01, 2017 Accepted: July 12, 2017 Published: July 20, 2017
ABSTRACT
In this study, we investigated the diagnostic potential of serum exosomal colorectal neoplasia differentially expressed (CRNDE-p) long coding RNA and microRNA-217 in colorectal carcinoma (CRC). We detected high CRNDE-p and low miR-217 levels in exosomes released by multiple CRC cell lines into culture media as well as in sera from CRC xenograft mice and CRC patients. Conversely, we observed lower CRNDE-p and higher miR-217 levels in serum exosomes from post-chemotherapy than from pre-chemotherapy patient samples. The area under curve (AUC) value for the serum exosomal CRNDE-p and miR-217 combination was higher than CRNDE-p or miR-217 alone. Moreover, high CRNDE-p and low miR-217 serum exosomal levels correlated with advanced clinical stages (III/IV), tumor classification (T3/T4), and lymph node or distant metastasis. Thus combined evaluation of serum exosomal CRNDE-p and miR-217 levels show diagnostic and prognostic potential for CRC patients.
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