Docking of CDK1 with antibiotic drugs revealed novel therapeutic value in breast ductal cancer in situ
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Zhong-Hai Ding1,*, Jia Qi2,*, An-Quan Shang3,4,*, Yu-Jie Zhang5,**, Jun Wei5,**, Li-Qing Hu6, Wei-Wei Wang7 and Man Yang8
1Department of Senior Cadres’ Healthcare, Jinling Hospital, Medical School of Nanjing University, Nanjing 210002, Jiangsu, China
2Department of Dermatology, Nanjing Medical University Affiliated Wuxi Second Hospital, Wuxi 214002, Jiangsu, China
3Department of Laboratory Medicine, Tongji Hospital of Tongji University, Shanghai 200092, Shanghai, China
4The Sixth People’s Hospital of Yancheng City, Yancheng 224005, Jiangsu, China
5Clinical Medicine School, Ningxia Medical University, Yinchuan 750004, Ningxia, China
6Department of Laboratory Medicine, The first Hospital of Ningbo City, Ningbo 315010, Zhejiang, China
7Department of Pathology, The First People’s Hospital of Yancheng City and The Sixth People’s Hospital of Yancheng City, Yancheng 224001, Jiangsu, China
8Department of Laboratory Medicine, TCM Hospital of Yancheng City Affiliated to Nanjing University of Chinese Medicine, Yancheng 224001, Jiangsu, China
*Zhong-Hai Ding, Jia Qi and An-Quan Shang are the first authors
**Yu-Jie Zhang and Jun Wei are the second authors
Wei-Wei Wang, email: firstname.lastname@example.org
Li-Qing Hu, email: email@example.com
Man Yang, email: firstname.lastname@example.org
Keywords: ductal cancer in situ, GEO, molecular signature, microarray, protein docking
Received: March 12, 2017 Accepted: May 07, 2017 Published: June 28, 2017
The aim of our research is to identify potential genes associated with Ductal carcinoma in situ (DCIS) through microarrays. The microarray dataset GS54665 were downloaded from the GEO(Gene Expression Omnibus) database. Dysregulated genes were screened and their associations with DCIS was analyzed by comprehensive bioinformatics tools. A total of 649 differential expression genes were identified between normal and DCIS samples, including 224 up-regulated genes and 425 down-regulated genes. Biological process annotation and pathway enrichment analysis identified several DCIS-related signaling pathways. Finally, PPI network was constructed with String website in order to get the hub codes involved in Ductal carcinoma in situ. We thus concluded that Five genes: CDK1, CCNB2, MAD2L1, PPARG, ACACB were finally identified to participate in the regulation and serve as potential diagnosis signatures in in Ductal carcinoma in situ. Finally, complmentarity between CDK1 and three drugs, Aminophenazone, Pomalidomide and the Rosoxacin, implies novel pharmacological value of those drugs in breast cancer.
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