Oncotarget

Research Papers:

Cationic liquid crystalline nanoparticles for the delivery of synthetic RNAi-based therapeutics

Emanuela Gentile _, Taro Oba, Jing Lin, Ruping Shao, Feng Meng, Xiaobo Cao, Heather Y. Lin, Majidi Mourad, Apar Pataer, Veerabhadran Baladandayuthapani, Dong Cai, Jack A. Roth and Lin Ji

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Oncotarget. 2017; 8:48222-48239. https://doi.org/10.18632/oncotarget.18421

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Abstract

Emanuela Gentile1, Taro Oba1, Jing Lin1, Ruping Shao1, Feng Meng1, Xiaobo Cao1, Heather Y. Lin2, Majidi Mourad1, Apar Pataer1, Veerabhadran Baladandayuthapani2, Dong Cai3, Jack A. Roth1 and Lin Ji1

1Section of Thoracic Molecular Oncology, Department of Thoracic & Cardiovascular Surgery, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States

2Department of Biostatistics, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, United States

3Department of Physics, The University of Houston, Houston, TX 77004, United States

Correspondence to:

Emanuela Gentile, email: egentile@mdanderson.org

Keywords: delivery systems, cationic nanoparticles, RNAi, lung cancer, gene silencing

Received: December 28, 2016     Accepted: May 05, 2017     Published: June 09, 2017

ABSTRACT

RNA interference (RNAi)-based therapeutics have been used to silence the expression of targeted pathological genes. Small interfering RNA (siRNAs) and microRNA (miRNAs) inhibitor have performed this function. However, short half-life, poor cellular uptake, and nonspecific distribution of small RNAs call for the development of novel delivery systems to facilitate the use of RNAi. We developed a novel cationic liquid crystalline nanoparticle (CLCN) to efficiently deliver synthetic siRNAs and miRNAs. CLCNs were prepared by using high-speed homogenization and assembled with synthetic siRNA or miRNA molecules in nuclease-free water to create CLCN/siRNA or miRNA complexes. The homogeneous and stable CLCNs and CLCN-siRNA complexes were about 100 nm in diameter, with positively charged surfaces. CLCNs are nontoxic and are taken up by human cells though endocytosis. Significant inhibition of gene expression was detected in transiently transfected lung cancer H1299 cells treated with CLCNs/anti-GFP complexes 24 hours after transfection. Biodistribution analysis showed that the CLCNs and CLCNs-RNAi complexes were successfully delivered to various organs and into the subcutaneous human lung cancer H1299 tumor xenografts in mice 24 hours after systemic administration. These results suggest that CLCNs are a unique and advanced delivery system capable of protecting RNAi from degradation and of efficiently delivering RNAi in vitro and in vivo.


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