Tissue and serum lipidome shows altered lipid composition with diagnostic potential in mycosis fungoides
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Chenchen Xu1,*, Dan Zhou2,*, Yixin Luo1, Shuai Guo2, Tao Wang1, Jie Liu1, Yuehua Liu1 and Zhili Li2
1Department of Dermatology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China
2Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China
*These authors have contributed equally to this work and should be considered co-first authors
Jie Liu, email: Liujie04672@pumch.cn
Yuehua Liu, email: firstname.lastname@example.org
Zhili Li, email: email@example.com
Keywords: cutaneous T cell lymphoma, mycosis fungoides, mass spectrometry imaging, matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry, lipidomics
Received: December 23, 2016 Accepted: May 04, 2017 Published: May 26, 2017
Mycosis fungoides (MF) is the most common type of cutaneous T cell lymphoma. In this study, we used matrix-assisted laser desorption/ionization-Fourier transform ion cyclotron resonance mass spectrometry (MALDI-FTICR–MS) to perform lipidomic profiling of 5 MF tissue samples and 44 serum samples (22 from MF patients and 22 from control subjects). Multivariate statistical analysis of the mass spectral data showed that MF tissues had altered levels of seven lipids and MF sera had altered levels of twelve. Among these, six phosphotidylcholines, PC (34:2), PC (34:1), PC (36:3), PC (36:2), PC (32:0), and PC (38:4) and one sphingomyelin, SM (16:0) were altered in both MF tissues and sera. PC (34:2), PC (34:1), PC (36:3), and PC (36:2) levels were increased in both tissues and sera from MF patients, whereas SM (16:0), PC (32:0), and PC (38:4) levels were increased in MF sera but were decreased in MF tissues. We have thus identified multiple lipids that are altered in MF tissues and sera. This suggests serological and tissue lipidomic profiling could be an effective approach to screening for diagnostic biomarkers of MF.
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