Oncotarget

Research Papers:

Identification of hMex-3A and its effect on human bladder cancer cell proliferation

Ying Huang, Chao Fang, Jing-Wen Shi, Yu Wen and Da Liu _

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Oncotarget. 2017; 8:61215-61225. https://doi.org/10.18632/oncotarget.18050

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Abstract

Ying Huang1, Chao Fang1, Jing-Wen Shi1, Yu Wen2 and Da Liu3

1Department of Ultrasound, Shengjing Hospital of China Medical University, Shenyang 110004, China

2Department of Histoembryology, China Medical University, Shenyang 110000, China

3Department of Orthopedics, Shengjing Hospital of China Medical University, Shenyang 110004, China

Correspondence to:

Da Liu, email: spinecmu@163.com

Keywords: hMex-3A, RNA-binding protein, cell proliferation, bladder cancer

Received: January 10, 2017     Accepted: April 25, 2017     Published: May 22, 2017

ABSTRACT

In this study, hMex-3A was selected from TCGA database as a research object to observe the effects of small interfering RNA (siRNA) targeting hMex-3A on the biological activities of human bladder cancer and explore its mechanism for the first time. In this study, there were 2 groups including negative control group and hMex-3A-siRNA-transfected cells group for 5637 and T24 cell lines, respectively. After bladder cancer cells were transfected with the interference RNA sequence, proliferation of transfected cells were assessed by Celigo Cell Counting, and apoptosis were detected by flow cytometry. The knockdown rate of hMex-3A was 74% in 5637 cells and 68% in T24 cells after RNA interference. In addition, Celigo Cell Counting indicated that cell viability was significantly lower in hMex-3A-siRNA-transfected cells group (2196/well) than in negative control group (6777/well) (P < 0.05), but T24 cells did not show statistical significance between hMex-3A-siRNA-transfected cells group (5799/well) and negative control group (7899/well) (P >0.05). Flow cytometer showed that apoptosis was the highest and cells were significantly blocked after cells were transfected in hMex-3A-siRNA-transfected cells group in 5 days later (P < 0.05). Mex-3A protein was detected in bladder carcinoma sections with a mean staining intensity of 7.06±2.60. Mex-3A protein expression was significantly higher in cancerous tissue than in para-cancerous tissue (P <0.05). Our study suggested that siRNA targeting hMex-3A could markedly inhibit cell proliferation and promote apoptosis in 5637 cells. These might have significant implications to bladder carcinogenesis and serve as a potential target for the treatment of bladder cancer.


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