Oncotarget

Research Papers:

The effects of blueberry anthocyanins on histone acetylation in rat liver fibrosis

Wei Zhan, Xin Liao, Ru-Jia Xie, Tian Tian, Lei Yu, Xing Liu, Jing Liu, Po Li, Bing Han _, Ting Yang, Bei Zhang, Li-Jun Cai, Rui Li and Qin Yang

PDF  |  HTML  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:96761-96773. https://doi.org/10.18632/oncotarget.17842

Metrics: PDF 746 views  |   HTML 1384 views  |   ?  


Abstract

Wei Zhan1,*, Xin Liao2,*, Ru-Jia Xie3,*, Tian Tian3, Lei Yu3, Xing Liu3, Jing Liu2, Po Li4, Bing Han3, Ting Yang3, Bei Zhang5, Li-Jun Cai6, Rui Li6 and Qin Yang3

1General Surgery of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

2Imaging Department of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

3Department of Physiology of The Guizhou Medical University, Guiyang 550004, Guizhou Province, China

4Department of Pathology of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

5Ultrasonic Center of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

6Department of Neurology of The Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Guizhou Province, China

7Department of Recovery of the Guizhou People’s Hospital, Guiyang 550004, Guizhou Province, China

*Co-first authors

Correspondence to:

Bing Han, email: hanbing_2016a@163.com

Qin Yang, email: yangqin_2016a@163.com

Keywords: blueberry, anthocyanin, hepatic stellate cell, liver fibrosis, histone acetylation

Received: January 18, 2017    Accepted: April 17, 2017    Published: May 12, 2017

ABSTRACT

To determine the effects ofanthocyanins from blueberries on hepatic stellate cell (HSCs-T6) and on histone acetylation during liver fibrosis induced by CCl4 in rats. Fifty male SD rats weighing 180 ± 20g were randomly placed into a control group, a hepatic fibrosis group, a blueberry treatment group, a blueberry intervention group, and a natural recovery group. After the rats were sacrificed, the livers and the liver indexes were measured, and the pathological changes were observed by HE staining and Masson staining. The blood was analyzed for the four indexes of liver fibrosis and liver function; nucleoprotein from liver tissues and karyoplasm were isolated to determine the expression of acH3K9, acH3K14, and acH3K18 by Western blotting.

Compared with the lethal rate of the control group, the median lethal rate of HSCs-T6 cells treated with a the 50μmol/L concentration was 66.94% (P < 0.05). The protein expression on α-SMA, type I collagen, TIMP1 significantly decreased (P < 0.05) following treatment with 50 ug/ml of anthocyanin for 36 h; moreover, the expression of acH3K9, acH3K14 and acH3K18 modification were up-regulated (P < 0.05). Furthermore, compared with the liver in the model group, the liver in the intervention group showed the most obvious improvement (P < 0.01), and its karyoplasm had increased expression of acH3K9, acH3K14 and acH3K18 (P<0.01).

Regulating histone acetylation could improve liver function and liver fibrosis indexes in rats with hepatic fibrosis. The mechanism might be related to certain genes that promote apoptosis, so as to inhibit the effect of anti hepatic fibrosis.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 17842