Nac1 promotes self-renewal of embryonic stem cells through direct transcriptional regulation of c-Myc
Metrics: PDF 626 views | HTML 1320 views | ?
Yan Ruan1,2,*, Jianrong He1,3,*, Wei Wu4,*, Ping He4,*, Yanping Tian1, Lan Xiao1, Gaoke Liu1, Jiali Wang1, Yuda Cheng1, Shuo Zhang1, Yi Yang5, Jiaxiang Xiong5, Ke Zhao6, Ying Wan2, He Huang3, Junlei Zhang1 and Rui Jian1
1Laboratory of Stem Cell and Developmental Biology, Department of Histology and Embryology, Third Military Medical University, Chongqing 400038, China
2Biomedical Analysis Center, Third Military Medical University, Chongqing 400038, China
3Department of Anesthesiology, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
4Department of Cardiothoracic Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China
5Experimental Center of Basic Medicine, College of Basic Medical Sciences, Third Military Medical University, Chongqing 400038, China
6State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 100850, China
*These authors contributed equally to this work
Ying Wan, email: firstname.lastname@example.org
He Huang, email: email@example.com
Junlei Zhang, email: firstname.lastname@example.org
Keywords: embryonic stem cells, Nac1, c-Myc, transcriptional regulation, self-renewal
Received: March 10, 2017 Accepted: April 27, 2017 Published: May 10, 2017
The pluripotency transcriptional network in embryonic stem cells (ESCs) is composed of distinct functional units including the core and Myc units. It is hoped that dissection of the cellular functions and interconnections of network factors will aid our understanding of ESC and cancer biology. Proteomic and genomic approaches have identified Nac1 as a member of the core pluripotency network. However, previous studies have predominantly focused on the role of Nac1 in psychomotor stimulant response and cancer pathogenesis. In this study, we report that Nac1 is a self-renewal promoting factor, but is not required for maintaining pluripotency of ESCs. Loss of function of Nac1 in ESCs results in a reduced proliferation rate and an enhanced differentiation propensity. Nac1 overexpression promotes ESC proliferation and delays ESC differentiation in the absence of leukemia inhibitory factor (LIF). Furthermore, we demonstrated that Nac1 directly binds to the c-Myc promoter and regulates c-Myc transcription. The study also revealed that the function of Nac1 in promoting ESC self-renewal appears to be partially mediated by c-Myc. These findings establish a functional link between the core and c-Myc-centered networks and provide new insights into mechanisms of stemness regulation in ESCs and cancer.
All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.