Correction: Suppression of CD300A inhibits the growth of diffuse large B-cell lymphoma
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Present: Due to an error during manuscript preparation, the primer sequences for CD300A included in the published paper are incorrect.
Correct: The proper primer sequences are shown below. The authors sincerely apologize for this oversight.
Original article: Oncotarget. 2015; 6:31191-202. DOI: 10.18632/oncotarget.5152.
Quantitative real-time PCR (qRT-PCR)
The levels of CD300A mRNA was determined using qRT-PCR as previously described . Brie y, total RNA was extracted using Trizol reagent (Invitrogen, Shanghai, China) according to the manufacturer’s instructions. cDNA was prepared using a reverse transcription kit (Thermo Scientific, Shanghai, China). qRT-PCR was performed using SYBR Green PCR master mix on a LightCycler 480 system (Roche, Shanghai, China). All samples were run in triplicate. The levels of CD300A expression were normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). The primers used for CD300A were 5’-GGTCCCAGCATCAACGTCAA-3’ (forward) and 5’-CCCACTGCAAACAGGGTAGT-3’ (reverse), and for GAPDH were 5’-CGACCACTTTGTCAAGCTCA-3’ (forward) and 5’-CCCTGTTGCTGTAGCCAAAT-3’ (reverse).
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