High expression of miR-493-5p positively correlates with clinical prognosis of non small cell lung cancer by targeting oncogene ITGB1
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Zhu Liang1, Rui Kong2, Zhan He1, Li-Yao Lin1, Shan-Shan Qin3, Chun-Yuan Chen1, Zhan-Qiang Xie1, Fei Yu3, Guo-Qian Sun1, Chun-Guang Li4, Da Fu3, Geng-Xi Jiang4, Jie Chen1 and Yu-Shui Ma3,5
1Department of Cardiothoracic Surgery, The Affiliated Hospital of Guangdong Medical University, Zhanjiang 524001, China
2Medical College of Soochow University, Soochow 215006, China
3Department of Nuclear Medicine, Shanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200072, China
4Department of Thoracic Surgery, Changhai Hospital of Second Military Medical University, Shanghai 200433, China
5Shanghai Engineering Research Center of Molecular Therapeutics and New Drug Development, College of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200062, China
Geng-Xi Jiang, email: firstname.lastname@example.org
Jie Chen, email: email@example.com
Yu-Shui Ma, email: firstname.lastname@example.org
Keywords: ITGB1, miR-493-5p, biomarker, survival, target
Received: February 17, 2017 Accepted: April 19, 2017 Published: May 07, 2017
Increasing evidence supports that microRNA (miRNA)-mediated gene regulation plays a significant functional role in cancer progression. To investigate the expression and clinical significance of ITGB1 in non small cell lung cancer (NSCLC), the expression levels of ITGB1 in NSCLC tissues and human normal lung tissues were analyzed in silico using genes microarray, KEGG pathway and hierarchical clustering analysis followed by validation with quantitative RT-PCR. Our results showed that ITGB1 was upregulated in NSCLC tissues when compared with normal lung tissues. Survival analysis based on the qRT-PCR data established that ITGB1 expression was attentively related to the prognosis of NSCLC, and patients with higher ITGB1 expression had shorter overall survival (OS). Moreover, ITGB1 was confirmed to be a direct target of miR-493-5p. Furthermore, concomitant high expression of ITGB1 and low expression of miR-493-5p correlated with a shorter median OS and PFS in NSCLC patients. In conclusion, our results provide the first evidence that ITGB1 is a direct target of miR-493-5p suggesting that ITGB1 and miR-493-5p may have potential prognostic value and may be useful as tumor biomarkers for the diagnosis of NSCLC patients.
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