Oncotarget

Research Papers:

Aquaporins 1, 3 and 8 expression in irritable bowel syndrome rats’ colon via NF-κB pathway

Guanqun Chao and Shuo Zhang _

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Oncotarget. 2017; 8:47175-47183. https://doi.org/10.18632/oncotarget.17565

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Abstract

Guanqun Chao1 and Shuo Zhang2

1Department of Family Medicine, Sir Run Run Shaw Hospital, Zhejiang University, Hangzhou, China

2Department of Gastroenterology, The First Affiliated Hospital, Zhejiang Chinese Medical University, Hangzhou, China

Correspondence to:

Shuo Zhang, email: zhangshuotcm@163.com

Keywords: irritable bowel syndrome, aquaporins, NF-κB

Received: November 04, 2016     Accepted: April 18, 2017     Published: May 02, 2017

ABSTRACT

Objective: Our research was to detect the expression of aquaporins. NF-κB in Irritable bowel syndrome (IBS) rat models’ colon so as to find novel pathogenesisof IBS.

Results: The expression of AQP1, AQP3, and AQP8 of IBS model group was down-regulated while NF-κB p65 was up-regulated comparing with control group (p < 0.05), and the expression of AQP1, AQP3, and AQP8 of inhibitor group was up-regulated while NF-κB p65 was down-regulated comparing with IBS model group (p < 0.05).

Materials and Methods: 18 adult female SD big rats were divided into three groups:the rats in control group were normal rats,the rats in IBS model group and the rats of inhibitor group were injected with the inhibitor of NF-κB (PDTC). Immunohistochemical technique and western blot were performed to detect the expression of AQP1, AQP3, AQP8 and NF-κB p65. RT-PCR was performed to detect the expression of AQP1, AQP3, and AQP8.

Conclusions: Liquid water metabolic abnormalities and intestine permeability alteration might be the mechanism of IBS by down-regulating AQP1, AQP3 and AQP8 via NF-κB pathway.


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