Oncotarget

Research Papers:

Perfluorodecanoic acid (PFDA) promotes gastric cell proliferation via sPLA2-IIA

Tianyi Dong, Yanping Peng, Ning Zhong, Fengyan Liu, Hanyu Zhang, Mengchen Xu, Rutao Liu, Mingyong Han, Xingsong Tian, Jihui Jia, Lap Kam Chang, Liang-Hong Guo and Shili Liu _

PDF  |  HTML  |  Supplementary Files  |  How to cite  |  Order a Reprint

Oncotarget. 2017; 8:50911-50920. https://doi.org/10.18632/oncotarget.17284

Metrics: PDF 586 views  |   HTML 1136 views  |   ?  


Abstract

Tianyi Dong1,2,*, Yanping Peng1,*, Ning Zhong3, Fengyan Liu3, Hanyu Zhang1, Mengchen Xu4, Rutao Liu4, Mingyong Han5, Xingsong Tian2, Jihui Jia1, Lap Kam Chang1, Liang-Hong Guo6 and Shili Liu1

1School of Medicine, Shandong University, Jinan, Shandong, 250012, China

2Department of Breast Thyroid Surgery, Shandong Provincial Hospital, Shandong University, Jinan, Shandong, 250021, China

3Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, 250012, China

4School of Environmental Science and Engineering, Shandong University, Jinan, Shandong, 250100, China

5Cancer Therapy and Research Center, Shandong Provincial Hospital, Shandong university, Jinan, Shandong 250021, China

6State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China

*These authors contributed equally to this work

Correspondence to:

Liang-Hong Guo, email: lhguo@rcees.ac.cn

Shili Liu, email: liushili@sdu.edu.cn

Keywords: PFDA, proliferation, sPLA2-IIA, TCF4

Received: September 06, 2016     Accepted: April 05, 2017     Published: April 20, 2017

ABSTRACT

The association of perfluorodecanoicacid (PFDA) with tumor promotion and associated effects is not clear. Given that PDFA is mostly consumed with food and drinking water, we evaluated the effects of PFDA on a gastric cell line. When added to cell cultures, PFDA significantly increased growth rate and colony forming ability compared with control treatment. We found that suppression of cell senescence, but not apoptosis or autophagy was associated with PFDA-induced promotion of cell amount. To determine the molecular mechanism that was involved, DNA microarray assays was used to analyze changes in gene expression in response to PFDA treatment. Data analysis demonstrated that the vascular endothelial growth factor signaling pathway had the lowest p-value, with sPLA2-IIA (pla2g2a) exhibits the most altered expression pattern within the pathway. Moreover, sPLA2-IIA and its transcription factor TCF4, known as a direct target and a binding partner of Wnt/β-catenin signaling in gastric cells respectively, were the third and second most varied genes globally. Cells transfected with expression plasmids pENTER-tcf4 and pENTER-pla2g2a show reduced cell proliferation by more than 60% and 30% respectively. Knockdown with sPLA2-IIA siRNA provided additional evidence that sPLA2-IIA was a mediator of PFDA-induced cell senescence suppression. The results suggest for the first time that PFDA induced suppression of cell senescence through inhibition of sPLA2-IIA protein expression and might increased the proliferative capacity of an existing tumor.


Creative Commons License All site content, except where otherwise noted, is licensed under a Creative Commons Attribution 3.0 License.
PII: 17284