Oncotarget

Research Papers:

Correlation between microRNA-143 in peripheral blood mononuclear cells and disease severity in patients with psoriasis vulgaris

Yi-Zhi Zheng, Chun-Feng Chen _, Li-Ying Jia, Tu-Gen Yu, Jie Sun and Xiao-Yong Wang

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Oncotarget. 2017; 8:51288-51295. https://doi.org/10.18632/oncotarget.17260

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Abstract

Yi-Zhi Zheng1, Chun-Feng Chen2, Li-Ying Jia1, Tu-Gen Yu1, Jie Sun1 and Xiao-Yong Wang3

1Department of Dermatology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, P.R. China

2Department of Mental Health, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou 310006, P.R. China

3Department of Dermatology, Hangzhou Traditional Chinese Medical Hospital, Hangzhou 310007, P.R. China

Correspondence to:

Chun-Feng Chen, email: chenchunff233@163.com

Keywords: disease severity, microRNA-143, psoriasis vulgaris, peripheral blood mononuclear cell

Received: August 26, 2016    Accepted: January 10, 2017    Published: April 20, 2017

ABSTRACT

This study aims to explore the correlation between microRNA-143 (miR-143) expression in peripheral blood mononuclear cells (PBMCs) and disease severity in patients with psoriasis vulgaris. From March 2014 to November 2015, 194 patients with psoriasis vulgaris (102 patients in progressive stage and 92 patients in stable stage) were selected as the case group and 175 healthy people as a control group were enrolled in this study. ELISA was used to detect the levels of IL-17 and VEGF in serum. The qRT-PCR assay was performed to detect the relative expression of miR-143 in PBMCs. Disease severity in psoriasis vulgaris patients was graded with Psoriasis Lesions Area and Severity Index (PASI). The value of miR-143 expression in PBMCs for the diagnosis of psoriasis vulgaris was evaluated using receiver operating characteristic (ROC) curve. The correlation between miR-143 expression in PBMCs and PASI scores was measured using Spearman rank correlation analysis. Compared with the control group, serum levels of IL-17 and VEGF were higher and miR-143 expression in PBMCs was lower in the case group. Furthermore, miR-143 expression in PBMCs was lower in patients in progressive stage than that in patients with stable stage. The relative expression of miR-143 in PBMCs was negatively correlated with PASI scores of patients with psoriasis vulgaris. ROC curve showed that miR-143 was a reliable and accurate biomarker of psoriasis vulgaris. Our findings suggest that miR-143 expression in PBMCs is negatively correlated the disease severity in psoriasis vulgaris.


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