Oncotarget

Research Papers:

Upregulation of calprotectin in mild IgE-mediated ovalbumin hypersensitivity

Qingling Zhu, Feng Li, Junli Wang, Jingqiu Ma and Xiaoyang Sheng _

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Oncotarget. 2017; 8:37342-37354. https://doi.org/10.18632/oncotarget.16954

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Abstract

Qingling Zhu1,*, Feng Li1,*, Junli Wang1, Jingqiu Ma1, Xiaoyang Sheng1

1Department of Child and Adolescent Healthcare, MOE-Shanghai Key Laboratory of Children’s Environmental Health, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, 200092, Shanghai, China

*These authors contributed equally to this work

Correspondence to:

Xiaoyang Sheng, email: shengxiaoyangcn@aliyun.com

Keywords: calprotectin, S100A8/A9, food hypersensitivity, IgE, animal experiment

Received: December 07, 2016     Accepted: March 27, 2017     Published: April 08, 2017

ABSTRACT

Calprotectin, also known as S100A8/A9, has been linked to gut inflammation caused by IgE-mediated food hypersensitivities, but the pathophysiologic abnormalities it causes remain to be determined. We created a mild food hypersensitivity model through oral gavage of ovalbumin in Norway brown rats without using immune adjuvant. Changes in the levels of calprotectin and inflammation-associated cytokines were then observed over time. We found that fecal calprotectin as well as jejunal and liver TLR4, TNF-α, NF-κB, IL-1β, and IL-6 were upregulated in hypersensitive rats. Additionally, the influence of calprotectin on CD4+ T and dendritic cells was observed by co-culturing CD4+ T cells with dendritic cells, which revealed a shift toward increased Th2 T cells in calprotectin-treated cultures. These results suggest that calprotectin, along with other inflammatory factors, promotes the inflammation seen in mild food allergy.


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