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Research Papers:

Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus

Zhen-Yu She, Meng-Ying Pan, Fu-Qing Tan and Wan-Xi Yang _

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Oncotarget. 2017; 8:36469-36483. https://doi.org/10.18632/oncotarget.16863

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Abstract

Zhen-Yu She1, Meng-Ying Pan1, Fu-Qing Tan2, Wan-Xi Yang1

1The Sperm Laboratory, College of Life Sciences, Zhejiang University, Hangzhou 310058, China

2The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China

Correspondence to:

Wan-Xi Yang, email: wxyang@spermlab.org

Keywords: kinesin-14, KIFC1, the Golgi apparatus, microtubule, the Golgi architecture

Received: December 16, 2016     Accepted: March 27, 2017     Published: April 05, 2017

ABSTRACT

The Golgi apparatus is the central organelle along the eukaryotic secretory and endocytic pathway. In non-polarized mammalian cells, the Golgi complex is usually located proximal to the nucleus at the cell center and is closely associated with the microtubule organizing center. Microtubule networks are essential in the organization and central localization of the Golgi apparatus, but the molecular basis underlying these processes are poorly understood. Here we reveal that minus end-directed kinesin-14 KIFC1 proteins are required for the structural integrity and positioning of the Golgi complex in non-polarized mammalian cells. Remarkably, we found that the motor domain of kinesin-14 KIFC1 regulates the recognition and binding of the Golgi and KIFC1 also statically binds to the microtubules via its tail domain. These findings reveal a new stationary binding model that kinesin-14 KIFC1 proteins function as crosslinkers between the Golgi apparatus and the microtubules and contribute to the central positioning and structural maintenance of the Golgi apparatus.


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PII: 16863