Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells
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Xiao-Yi Chen1,*, San-Zhong Xu2,*, Xuan-Wei Wang2,*, Xian-Yan Yang3, Liang Ma3, Lei Zhang4, Guo-Jing Yang4, Fan Yang1, Lin-Hong Wang1, Xin-Li Zhang5, Kang Ting5, Chang-You Gao3, Xiao-Zhou Mou1, Zhong-Ru Gou3 and Hai Zou6
1Clinical Research Institute, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China
2The First Affiliated Hospital, School of Medicine of Zhejiang University, Hangzhou 310009, China
3Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China
4Rui’an People’s Hospital & The 3rd Affiliated Hospital to Wenzhou Medical University, Rui’an 325005, China
5Dental and Craniofacial Research Institute, School of Dentistry, University of California, Los Angeles, CA 90095, USA
6Department of Cardiology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China
*These authors have contributed equally to this work
Xiao-Zhou Mou, email: firstname.lastname@example.org
Zhong-Ru Gou, email: email@example.com
Hai Zou, email: firstname.lastname@example.org
Keywords: mineral micronutrients, microparticles, osteogenic differentiation, rOMSCs, rMSCs
Received: January 02, 2017 Accepted: March 06, 2017 Published: March 28, 2017
Osteoporosis is a disease characterized by structural deterioration of bone tissue, leading to skeletal fragility with increased fracture risk. Calcium phosphates (CaPs) are widely used in bone tissue engineering strategies as they have similarities to bone apatite except for the absence of trace elements (TEs) in the CaPs. Bioactive glasses (BGs) have also been used successfully in clinic for craniomaxillofacial and dental applications during the last two decades due to their excellent potential for bonding with bone and inducing osteoblastic differentiation. In this study, we evaluated the osteogenic effects of the ionic dissolution products of the quaternary Si-Sr-Zn-Mg-codoped CaP (TEs-CaP) or 45S5 Bioglass® (45S5 BG), both as mixtures and separately, on rat bone marrow-derived mesenchymal stem cells (rOMSCs & rMSCs) from osteoporotic and normal animals, using an MTT test and Alizarin Red S staining. The materials enhanced cell proliferation and osteogenic differentiation, especially the combination of the BG and TEs-CaP. Analysis by quantitative PCR and ELISA indicated that the expression of osteogenic-specific genes and proteins were elevated. These investigations suggest that the TEs-CaP and 45S5 BG operate synergistically to create an extracellular environment that promotes proliferation and terminal osteogenic differentiation of both osteoporotic and normal rMSCs.
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