Oncotarget

Research Papers:

Systematic comparison of biologically active foreign ions-codoped calcium phosphate microparticles on osteogenic differentiation in rat osteoporotic and normal mesenchymal stem cells

Xiao-Yi Chen, San-Zhong Xu, Xuan-Wei Wang, Xian-Yan Yang, Liang Ma, Lei Zhang, Guo-Jing Yang, Fan Yang, Lin-Hong Wang, Xin-Li Zhang, Kang Ting, Chang-You Gao, Xiao-Zhou Mou _, Zhong-Ru Gou and Hai Zou

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Oncotarget. 2017; 8:36578-36590. https://doi.org/10.18632/oncotarget.16618

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Abstract

Xiao-Yi Chen1,*, San-Zhong Xu2,*, Xuan-Wei Wang2,*, Xian-Yan Yang3, Liang Ma3, Lei Zhang4, Guo-Jing Yang4, Fan Yang1, Lin-Hong Wang1, Xin-Li Zhang5, Kang Ting5, Chang-You Gao3, Xiao-Zhou Mou1, Zhong-Ru Gou3 and Hai Zou6

1Clinical Research Institute, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China

2The First Affiliated Hospital, School of Medicine of Zhejiang University, Hangzhou 310009, China

3Zhejiang-California International Nanosystems Institute, Zhejiang University, Hangzhou 310058, China

4Rui’an People’s Hospital & The 3rd Affiliated Hospital to Wenzhou Medical University, Rui’an 325005, China

5Dental and Craniofacial Research Institute, School of Dentistry, University of California, Los Angeles, CA 90095, USA

6Department of Cardiology, Zhejiang Provincial People’s Hospital, Hangzhou 310014, China

*These authors have contributed equally to this work

Correspondence to:

Xiao-Zhou Mou, email: mouxz@zjheart.com

Zhong-Ru Gou, email: zhrgou@zjheart.com

Hai Zou, email: haire1993@163.com

Keywords: mineral micronutrients, microparticles, osteogenic differentiation, rOMSCs, rMSCs

Received: January 02, 2017    Accepted: March 06, 2017    Published: March 28, 2017

ABSTRACT

Osteoporosis is a disease characterized by structural deterioration of bone tissue, leading to skeletal fragility with increased fracture risk. Calcium phosphates (CaPs) are widely used in bone tissue engineering strategies as they have similarities to bone apatite except for the absence of trace elements (TEs) in the CaPs. Bioactive glasses (BGs) have also been used successfully in clinic for craniomaxillofacial and dental applications during the last two decades due to their excellent potential for bonding with bone and inducing osteoblastic differentiation. In this study, we evaluated the osteogenic effects of the ionic dissolution products of the quaternary Si-Sr-Zn-Mg-codoped CaP (TEs-CaP) or 45S5 Bioglass® (45S5 BG), both as mixtures and separately, on rat bone marrow-derived mesenchymal stem cells (rOMSCs & rMSCs) from osteoporotic and normal animals, using an MTT test and Alizarin Red S staining. The materials enhanced cell proliferation and osteogenic differentiation, especially the combination of the BG and TEs-CaP. Analysis by quantitative PCR and ELISA indicated that the expression of osteogenic-specific genes and proteins were elevated. These investigations suggest that the TEs-CaP and 45S5 BG operate synergistically to create an extracellular environment that promotes proliferation and terminal osteogenic differentiation of both osteoporotic and normal rMSCs.


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