Oncotarget

Research Papers:

Glibenclamide, a diabetic drug, prevents acute radiation-induced liver injury of mice via up-regulating intracellular ROS and subsequently activating Akt–NF-κB pathway

Hu Liu, Shichao Wang, Zhao Wu, Ziyun Huang, Wei You Chen, Yanyong Yang, Jianguo Cui, Cong Liu, Hainan Zhao, Jiaming Guo, Pei Zhang, Fu Gao _, Bailong Li and Jianming Cai

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Oncotarget. 2017; 8:40568-40582. https://doi.org/10.18632/oncotarget.16501

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Abstract

Hu Liu1,*, Shichao Wang2,*, Zhao Wu2,*, Ziyun Huang2, Wei you Chen2, Yanyong Yang1, Jianguo Cui1, Cong Liu1, Hainan Zhao1, Jiaming Guo1, Pei Zhang1, Fu Gao1, Bailong Li1 and Jianming Cai1

1Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, China

2Fifth Cadet Battalion of Naval Medicine Department, Second Military Medical University, Shanghai, China

*These authors have contributed equally to this work

Correspondence to:

Fu Gao, email: gaofusmmu@163.com

Bailong Li, email: libailong2003@163.com

Jianming Cai, email: cjm882003@163.com

Keywords: acute radiation-induced liver injury, reactive oxygen species (ROS), ATP-sensitive potassium channel (KATP), membrane potential (MP), glibenclamide

Received: August 05, 2016    Accepted: February 15, 2017    Published: March 23, 2017

ABSTRACT

Background: Acute radiation-induced liver injury is a limitation for hepatoma radiotherapy. Come so far the clinical treatments are insufficient. The effective, specific, low toxicity and novel drugs are in powerful need. Glibenclamide is a common hypoglycemic. Some studies have revealed its relation with intracellular reactive oxygen species, the crucial mediator to radiation injury. This study is aimed to investigate if glibenclamide could act on the acute radiation-induced liver injury.

Results: Glibenclamide mitigated acute radiation-induced liver injury of mice, indicating as regression of hepatocellular edema, reduction of hepatic sinusoid, decline in serum ALP level and reduction of hepatocellular apoptosis. Pretreatment of glibenclamide reduced the radiosensitivity of NCTC-1469 cells. In mechanism, glibenclamide elevated cells membrane potential to up-regulate intracellular reactive oxygen species. The increased reactive oxygen species subsequently activated Akt–NF-κB pathway to promote survival of irradiated cells.

Methods: BALB/C male mice were intraperitoneal injected with glibenclamide 1 hour before hepatic irradiation. At designed time points the livers were taken to make histological study and bloods were collected to measure serum transaminase. With/without glibenclamide pretreatment the irradiated NCTC-1469 cells were tested apoptosis, viability and proliferation. By western blotting the involved molecules were detected.

Conclusions: Glibenclamide, prevents acute radiation-induced liver injury of mice via up-regulating intracellular reactive oxygen species and subsequently activating Akt–NF-κB pathway.


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