Research Papers:
Overexpression of Fli-1 in astrocytoma is associated with poor prognosis
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Abstract
Hung-Pei Tsai1, Tai-Hsin Tsai2,3, Ya-Ju Hsieh4, Yi-Ting Chen5, Chih-Ling Lee5, Yi-Cheng Tsai1, Ting-Chang She5, Chih-Lung Lin2,3, Chee-Yin Chai5,6,7, Aij-Lie Kwan2,3,8
1Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
2Division of Neurosurgery, Department of Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
3Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
4Department of Medical Imaging and Radiological Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
5Department of Pathology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
6Department of Pathology, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
7Institute of Biomedical Sciences, National Sun Yat-Sen University, Kaohsiung, Taiwan
8Department of Neurosurgery, University of Virginia, Charlottesville, VA, USA
Correspondence to:
Aij-Lie Kwan, email: [email protected]
Chee-Yin Chai, email: [email protected]
Keywords: astrocytoma, Fli-1, prognostic marker
Received: June 29, 2016 Accepted: February 20, 2017 Published: March 16, 2017
ABSTRACT
Background: Astrocytoma, a common and highly malignant type of brain tumor, is associated with poor overall survival despite advances in surgical treatment, radiotherapy, and chemotherapy. The nuclear transcription factor Fli-1 has been shown to increase cellular proliferation and tumorigenesis in many types of cancer; however, previous reports have not described a correlation between clinical outcomes and Fli-1 in astrocytoma patients. The present study aimed to elucidate the clinical role of Fli-1 in astrocytoma.
Results: High-level of Fli-1 protein expression was significantly association with World Health Organization (WHO) high grade and poor prognosis. A multivariate analysis revealed that the WHO grade and Fli-1 protein expression were independent factor of prognostic factors of patients with astrocytoma. In addition, Fli-1 silencing inhibited proliferation, migration, and invasion and led to the downregulation of Ki-67, VEGF, and cyclin D1 expression in the astrocytoma cells.
Materials and methods: Fli-1 protein expression in astrocytoma tissue samples were detected via immunohistochemistry, and potential correlations between clinical parameters and Fli-1 expression were assessed in patients with astrocytoma. Additionally, proliferation, invasion, and migration assays of astrocytoma cell lines were conducted to evaluate the effects of short interfering RNA (siRNA) on these processes; in addition, these cells were subjected to western blotting to detect the expression levels of Fli-1, Ki-67, VEGF, and Cyclin D1.
Conclusion: Fli-1 shows promise as a potential prognostic biomarker and therapeutic molecular target for astrocytoma patients.
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