Selenophosphate synthetase 1 (SPS1) is required for the development and selenium homeostasis of central nervous system in chicken (Gallus gallus)
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Jin-Long Li1,2,3,*, Wei Li1,2,3,*, Xue-Tong Sun1,2,3,*, Jun Xia1,2,3, Xue-Nan Li1,2,3, Jia Lin1,2,3, Cong Zhang1,2,3, Xiao-Chen Sun1,2,3, Shi-Wen Xu1,2,3
1College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China
2Heilongjiang Key Laboratory for Laboratory Animals and Comparative Medicine, Northeast Agricultural University, Harbin, 150030, P. R. China
3Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Northeast Agricultural University, Harbin, 150030, P. R. China
*These authors contributed equally to this work
Jin-Long Li, email: Jinlongli@neau.edu.cn
Shi-Wen Xu, email: firstname.lastname@example.org
Keywords: selenophosphate synthetase 1, selenium homeostasis, central nervous system, development, chicken
Received: February 14, 2017 Accepted: March 09, 2017 Published: March 16, 2017
Selenophosphate synthetase (SPS) is essential for selenoprotein biosynthesis. In two SPS paralogues, SPS1 was only cloned from a cDNA library prepared from avian organ. However, the biological function of SPS1 in chicken central nervous system (CNS) remains largely unclear. To investigate the role of avian SPS1 in the development and selenium (Se) homeostasis of CNS, fertile eggs, chicken embryos, embryo neurons and chicks were employed in this study. The response of SPS1 transcription to the development and Se levels of CNS tissues was analyzed using qRT-PCR. SPS1 gene exists extensively in the development of chicken CNS. The wide expression of avian SPS1 can be controlled by the Se content levels, which suggests that SPS1 is important in the regulation of Se homeostasis. The fundamental mechanism of these effects is that Se alters the half-life and stability of SPS1 mRNA. Therefore, SPS1 exerts an irreplaceable biological function in chicken CNS and Se homeostasis is closely related to the expression of SPS1. These results suggested that SPS1 was required for the development and Se homeostasis of CNS in chicken.
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