LncRNA Snhg1, a non-degradable sponge for miR-338, promotes expression of proto-oncogene CST3 in primary esophageal cancer cells
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Yan Yan1, Qingxia Fan1, Liping Wang1, Yue Zhou2, Jianhua Li3, Kun Zhou4
1Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
2Department of Medical Imaging, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
3Department of General Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
4Department of Thoracic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
Kun Zhou, email: firstname.lastname@example.org
Keywords: esophageal cancer, competing endogenous RNA, lncRNA Snhg1, miR-338, cystatin C
Received: October 04, 2016 Accepted: February 21, 2017 Published: March 14, 2017
Competing endogenous RNA (ceRNA) is a newly proposed mechanism that describes a crosstalk among lncRNAs, mRNAs and their shared miRNAs. In this study, the role of miR-338-3p (miR-338) in the progression of esophageal cancer and its involve in the ceRNA regulatory circuit lncRNA-Snhg1/CST3 were explored. MiR-338 displayed a 30% decreased expression in esophageal squamous cell carcinoma tissues compared with the adjacent. Then, proto-oncogene CST3 was predicted and validated as a target gene of miR-338. Gain-and-loss-function experiments indicated that miR-338 suppressed expression of CST3 protein (also Cystatin C, CysC), promoted expression of apoptotic proteins caspase-8/3, attenuated esophageal carcinoma cell growth and induced its apoptosis. In addition, lncRNA-Snhg1 was significantly upregulated in esophageal carcinoma tissues and promoted esophageal carcinoma cell growth. Furthermore, our results from bioinformatics, luciferase reporter gene and RNA pull-down assays indicated that Snhg1 could be directly bound by miR-338. Snhg1 acted as a non-degradable sponge to relieve the suppression on CST3 caused by miR-338. In conclusion, lncRNA-Snhg1 promoted cell proliferation by acting as a non-degradable sponge for the tumor suppressor miR-338 in esophageal cancer cells.
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