λ-Carrageenan improves the antitumor effect of dendritic cellbased vaccine
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Jinyao Li1,2,3, Adila Aipire2, Jinyu Li2, Hongge Zhu1, Yanping Wang4, Wenjia Guo1, Xiaoqin Li1, Jia Yang1, Chunling Liu1
1Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi 830011, China
2College of Life Science and Technology, Xinjiang University, Urumqi 830046, China
3XinJiang DingJu Biotech CO., LTD, Urumqi 830000, China
4Bayin Guoleng Vocational and Technical College, Korla 841000, China
Jinyao Li, email: firstname.lastname@example.org
Jinyu Li, email: email@example.com
Chunling Liu, email: firstname.lastname@example.org
Keywords: λ-carrageenan, TLR4, dendritic cell-based vaccine, cancer immunotherapy, cellular responses
Received: November 10, 2016 Accepted: January 24, 2017 Published: February 22, 2017
In this study, we investigated the effect of λ-carrageenan on the maturation and function of dendritic cells (DCs) and its adjuvant effect on DC-based vaccine. We found that λ-carrageenan dose-dependently decreased the endocytosis of DCs, promoted DC maturation and increased cytokine production through TLR4 mediated signaling pathway. λ-carrageenan treatment also enhanced the ability of DCs in the stimulating allogenic splenocyte proliferation. In TC-1 tumor mouse model, HPV peptides pulsed λ-carrageenan-DC (HPV-CGN-DC) significantly inhibited tumor growth compared with control group. The frequencies of CD4+ and CD8+ T cells in spleens of tumor mice and their activation status were significantly increased in HPV-CGN-DC group, but the frequencies of natural regulatory T cells and CD11b+Gr-1+ cells were significantly decreased. Further, HPV-CGN-DC induced strong CD8+ T cell responses, which are negatively correlated with tumor volumes. The results suggested that λ-carrageenan promoted DC maturation through TLR4 signaling pathway and could be used as the adjuvant in DC-based vaccines.
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